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Protective effects of Sapindus mukorossi Gaertn against fatty liver disease induced by high fat diet in rats

Protective effects of Sapindus mukorossi Gaertn against fatty liver disease induced by high fat diet in rats
Protective effects of Sapindus mukorossi Gaertn against fatty liver disease induced by high fat diet in rats

OBJECTIVES: Study the effects of alcohol extract of Sapindus mukorossi Gaertn (AESM) on the metabolism of blood fat, morphology of fenestrated liver sinusoidal endothelial cells (LSEC), and the ultrastructure of liver cells of the rats with non-alcoholic fatty liver disease (NAFLD).

METHODS: Divide SD rats into control group, model group, simvastatin (7.2 mg/kg) group, and S.mukorossi Gaertn group with high dosage (0.5 g/kg), moderate dosage (0.1 g/kg), and low dosage (0.05 g/kg). After feeding with fat-rich nutrients for 3 weeks and establishing the model of hepatic adipose, conduct intragastric administration and provide the rats with fat-rich nutrients at the same time. At the 43rd day, take blood sample and measure aminotransferase and different indexes of blood fat; take hepatic tissue for pathological section, and observe the hepatic morphological patterns under light microscope; obtain and fix the hepatic tissue after injecting perfusate into the body, and observe the changes of fenestrated LSEC under scanning electron microscope; observe the ultrastructure of liver cells under transmission electron microscope.

RESULTS: High-dosage alcohol extracts of S.mukorossi Gaertn can alleviate the AST, ALT, TC, TG, LDL, γ-GT, and ALP level, as well as raise the HDL and APN level in the serum of NAFLD-rat model. In addition, through the observation from light microscope and electron microscopes, the morphology of the hepatic tissue and liver cells as well as the recovery of the fenestrated LSEC in the treatment group has become normal.

CONCLUSIONS: Alcohol extracts of S.mukorossi Gaertn can regulate the level of blood fat and improve the pathological changes of the hepatic tissues in NAFLD-rat model, which demonstrates the effects of down-regulating fat level and protecting liver.

Animals, Dietary Fats/blood, Drugs, Chinese Herbal/therapeutic use, Fatty Liver/chemically induced, Female, Liver/metabolism, Male, Plant Extracts/therapeutic use, Rats, Rats, Sprague-Dawley, Sapindus/chemistry, Treatment Outcome
0006-291X
685-691
Peng, Qiuxian
4372d90b-6e6b-4daf-8181-32439b595e05
Zhang, Qin
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Xiao, Wei
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Shao, Meng
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Fan, Qin
2bcd9633-648d-4b56-8679-3733122303bb
Zhang, Hongwei
192ed3e8-f8cb-4a78-863f-6c09d9449607
Zou, Yukai
328b4fd9-da35-42bb-a032-b0a98ed33a2d
Li, Xin
50870cd7-3ec3-434e-a43a-b10d50e3d5b3
Xu, Wenxue
26f080fd-67ce-4f83-8c51-00c6e03af33a
Mo, Zhixian
54ef9e07-6c3c-4a24-8708-8e2a2b8bc9f4
Cai, Hongbing
0ebb0599-fa17-44c0-829e-8853c76e1b79
Peng, Qiuxian
4372d90b-6e6b-4daf-8181-32439b595e05
Zhang, Qin
30e6a53c-3302-4aac-ae77-42c8b8d8e4f1
Xiao, Wei
20758ef4-698c-46e5-bb9b-a97db7340379
Shao, Meng
2bc20c6d-f47a-493b-9085-0360a3e9a538
Fan, Qin
2bcd9633-648d-4b56-8679-3733122303bb
Zhang, Hongwei
192ed3e8-f8cb-4a78-863f-6c09d9449607
Zou, Yukai
328b4fd9-da35-42bb-a032-b0a98ed33a2d
Li, Xin
50870cd7-3ec3-434e-a43a-b10d50e3d5b3
Xu, Wenxue
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Mo, Zhixian
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Cai, Hongbing
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Peng, Qiuxian, Zhang, Qin, Xiao, Wei, Shao, Meng, Fan, Qin, Zhang, Hongwei, Zou, Yukai, Li, Xin, Xu, Wenxue, Mo, Zhixian and Cai, Hongbing (2014) Protective effects of Sapindus mukorossi Gaertn against fatty liver disease induced by high fat diet in rats. Biochemical and Biophysical Research Communications, 450 (1), 685-691. (doi:10.1016/j.bbrc.2014.06.035).

Record type: Article

Abstract

OBJECTIVES: Study the effects of alcohol extract of Sapindus mukorossi Gaertn (AESM) on the metabolism of blood fat, morphology of fenestrated liver sinusoidal endothelial cells (LSEC), and the ultrastructure of liver cells of the rats with non-alcoholic fatty liver disease (NAFLD).

METHODS: Divide SD rats into control group, model group, simvastatin (7.2 mg/kg) group, and S.mukorossi Gaertn group with high dosage (0.5 g/kg), moderate dosage (0.1 g/kg), and low dosage (0.05 g/kg). After feeding with fat-rich nutrients for 3 weeks and establishing the model of hepatic adipose, conduct intragastric administration and provide the rats with fat-rich nutrients at the same time. At the 43rd day, take blood sample and measure aminotransferase and different indexes of blood fat; take hepatic tissue for pathological section, and observe the hepatic morphological patterns under light microscope; obtain and fix the hepatic tissue after injecting perfusate into the body, and observe the changes of fenestrated LSEC under scanning electron microscope; observe the ultrastructure of liver cells under transmission electron microscope.

RESULTS: High-dosage alcohol extracts of S.mukorossi Gaertn can alleviate the AST, ALT, TC, TG, LDL, γ-GT, and ALP level, as well as raise the HDL and APN level in the serum of NAFLD-rat model. In addition, through the observation from light microscope and electron microscopes, the morphology of the hepatic tissue and liver cells as well as the recovery of the fenestrated LSEC in the treatment group has become normal.

CONCLUSIONS: Alcohol extracts of S.mukorossi Gaertn can regulate the level of blood fat and improve the pathological changes of the hepatic tissues in NAFLD-rat model, which demonstrates the effects of down-regulating fat level and protecting liver.

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More information

e-pub ahead of print date: 14 June 2014
Published date: 18 July 2014
Keywords: Animals, Dietary Fats/blood, Drugs, Chinese Herbal/therapeutic use, Fatty Liver/chemically induced, Female, Liver/metabolism, Male, Plant Extracts/therapeutic use, Rats, Rats, Sprague-Dawley, Sapindus/chemistry, Treatment Outcome

Identifiers

Local EPrints ID: 449759
URI: http://eprints.soton.ac.uk/id/eprint/449759
ISSN: 0006-291X
PURE UUID: 6f41f433-44ab-40fd-acad-c01515099345
ORCID for Wei Xiao: ORCID iD orcid.org/0000-0002-3898-1110
ORCID for Yukai Zou: ORCID iD orcid.org/0000-0002-9924-5926

Catalogue record

Date deposited: 16 Jun 2021 16:30
Last modified: 17 Jun 2021 02:01

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Contributors

Author: Qiuxian Peng
Author: Qin Zhang
Author: Wei Xiao ORCID iD
Author: Meng Shao
Author: Qin Fan
Author: Hongwei Zhang
Author: Yukai Zou ORCID iD
Author: Xin Li
Author: Wenxue Xu
Author: Zhixian Mo
Author: Hongbing Cai

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