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Identifying genomic regions targeted during eggplant domestication using transcriptome data

Identifying genomic regions targeted during eggplant domestication using transcriptome data
Identifying genomic regions targeted during eggplant domestication using transcriptome data
Identifying genes and traits that have diverged during domestication provides key information of importance for maintaining and even increasing yield and nutrients in existing crops. A ‘bottom up’ population genetics approach was used to identify signatures of selection across the eggplant genome, to better understand the process of domestication. RNA-seq data was obtained for four wild eggplants (Solanum insanum L.) and 16 domesticated eggplants (S. melongena L.) and mapped to the eggplant genome. SNPs exhibiting signatures of selection in domesticates were identified as those exhibiting high FST between the two populations (evidence of significant divergence) and low π for the domesticated population (indicative of a selective sweep). Some of these regions appear to overlap with previously identified QTL for domestication traits. Genes in regions of linkage disequilibrium surrounding these SNPs were searched against the Arabidopsis thaliana and tomato genomes to find orthologues. Subsequent Gene Ontology (GO) enrichment analysis identified over-representation of GO terms related to photosynthesis and response to the environment. This work reveals genomic changes involved in eggplant domestication and improvement, and how this compares to observed changes in the tomato genome, revealing shared chromosomal regions involved in the domestication of both species.
0022-1503
Page, Anna
17800ba3-c0b8-4ddf-b78c-94273b67189c
Chapman, Mark
8bac4a92-bfa7-4c3c-af29-9af852ef6383
Page, Anna
17800ba3-c0b8-4ddf-b78c-94273b67189c
Chapman, Mark
8bac4a92-bfa7-4c3c-af29-9af852ef6383

Page, Anna and Chapman, Mark (2021) Identifying genomic regions targeted during eggplant domestication using transcriptome data. Journal of Heredity, [esab035]. (doi:10.1093/jhered/esab035).

Record type: Article

Abstract

Identifying genes and traits that have diverged during domestication provides key information of importance for maintaining and even increasing yield and nutrients in existing crops. A ‘bottom up’ population genetics approach was used to identify signatures of selection across the eggplant genome, to better understand the process of domestication. RNA-seq data was obtained for four wild eggplants (Solanum insanum L.) and 16 domesticated eggplants (S. melongena L.) and mapped to the eggplant genome. SNPs exhibiting signatures of selection in domesticates were identified as those exhibiting high FST between the two populations (evidence of significant divergence) and low π for the domesticated population (indicative of a selective sweep). Some of these regions appear to overlap with previously identified QTL for domestication traits. Genes in regions of linkage disequilibrium surrounding these SNPs were searched against the Arabidopsis thaliana and tomato genomes to find orthologues. Subsequent Gene Ontology (GO) enrichment analysis identified over-representation of GO terms related to photosynthesis and response to the environment. This work reveals genomic changes involved in eggplant domestication and improvement, and how this compares to observed changes in the tomato genome, revealing shared chromosomal regions involved in the domestication of both species.

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esab035 - Accepted Manuscript
Restricted to Repository staff only until 15 June 2022.
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e-pub ahead of print date: 15 June 2021

Identifiers

Local EPrints ID: 449916
URI: http://eprints.soton.ac.uk/id/eprint/449916
ISSN: 0022-1503
PURE UUID: 2172ad79-f18c-4836-b17d-341ca016ff92
ORCID for Mark Chapman: ORCID iD orcid.org/0000-0002-7151-723X

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Date deposited: 25 Jun 2021 16:31
Last modified: 26 Jun 2021 01:44

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Contributors

Author: Anna Page
Author: Mark Chapman ORCID iD

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