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Pre-adolescence DNA methylation is associated with BMI status change from pre- to post-adolescence

Pre-adolescence DNA methylation is associated with BMI status change from pre- to post-adolescence
Pre-adolescence DNA methylation is associated with BMI status change from pre- to post-adolescence

Background: previous studies have shown that DNA methylation (DNAm) is associated with body mass index (BMI). However, it is unknown whether DNAm at pre-adolescence is associated with BMI status transition from pre- to post-adolescence. In the Isle of Wight (IoW) birth cohort, genome-wide DNA methylation in whole blood was measured using Illumina Infinium Human450 and EPIC BeadChip arrays in n = 325 subjects, and pre- to post-adolescence BMI transition was classified into four groups: (1) normal to normal, (2) normal to overweight or obese, (3) overweight or obese to normal, and (4) persistent overweight or obese. We used recursive random forest to screen genome-wide Cytosine-phosphate-Guanine (CpG) sites with DNAm potentially associated with BMI transition for each gender, and the association of BMI status transition with DNAm at an earlier age was assessed via logistic regressions. To evaluate gender specificity, interactions between DNAm and gender were included in the model. Findings in the IoW cohort were further tested in an independent cohort, the Avon Longitudinal Study of Parents and Children (ALSPAC).

Results: in total, 174 candidate CpGs were selected including CpGs from screening and CpGs previously associated correctionally with BMI in children and adults. Of these 174 CpGs, pre-adolescent DNAm of 38 CpGs in the IoW cohort was associated with BMI status transition, including 30 CpGs showing gender-specific associations. Thirteen CpGs showed consistent associations between the IoW cohort and the ALSPAC cohort (11 of which were gender-specific).

Conclusion: pre-adolescence DNAm is associated with the change in BMI status from pre- to post-adolescence and such associations are likely to be gender-specific.

Adolescence, BMI status transition, Body mass index (BMI), DNA methylation, Longitudinal, Obesity
1868-7075
Wang, Jiajing
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Zhang, Hongmei
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Rezwan, Faisal I
203f8f38-1f5d-485b-ab11-c546b4276338
Relton, Caroline
7a9fe7f7-d14b-4bb7-be71-a3afa6ff8538
Arshad, S. Hasan
917e246d-2e60-472f-8d30-94b01ef28958
Holloway, John W
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Wang, Jiajing
6cccda97-d46f-406e-a26d-a63ce8750882
Zhang, Hongmei
9f774048-54d6-4321-a252-3887b2c76db0
Rezwan, Faisal I
203f8f38-1f5d-485b-ab11-c546b4276338
Relton, Caroline
7a9fe7f7-d14b-4bb7-be71-a3afa6ff8538
Arshad, S. Hasan
917e246d-2e60-472f-8d30-94b01ef28958
Holloway, John W
4bbd77e6-c095-445d-a36b-a50a72f6fe1a

Wang, Jiajing, Zhang, Hongmei, Rezwan, Faisal I, Relton, Caroline, Arshad, S. Hasan and Holloway, John W (2021) Pre-adolescence DNA methylation is associated with BMI status change from pre- to post-adolescence. Clinical Epigenetics, 13 (1), [64]. (doi:10.1186/s13148-021-01042-4).

Record type: Article

Abstract

Background: previous studies have shown that DNA methylation (DNAm) is associated with body mass index (BMI). However, it is unknown whether DNAm at pre-adolescence is associated with BMI status transition from pre- to post-adolescence. In the Isle of Wight (IoW) birth cohort, genome-wide DNA methylation in whole blood was measured using Illumina Infinium Human450 and EPIC BeadChip arrays in n = 325 subjects, and pre- to post-adolescence BMI transition was classified into four groups: (1) normal to normal, (2) normal to overweight or obese, (3) overweight or obese to normal, and (4) persistent overweight or obese. We used recursive random forest to screen genome-wide Cytosine-phosphate-Guanine (CpG) sites with DNAm potentially associated with BMI transition for each gender, and the association of BMI status transition with DNAm at an earlier age was assessed via logistic regressions. To evaluate gender specificity, interactions between DNAm and gender were included in the model. Findings in the IoW cohort were further tested in an independent cohort, the Avon Longitudinal Study of Parents and Children (ALSPAC).

Results: in total, 174 candidate CpGs were selected including CpGs from screening and CpGs previously associated correctionally with BMI in children and adults. Of these 174 CpGs, pre-adolescent DNAm of 38 CpGs in the IoW cohort was associated with BMI status transition, including 30 CpGs showing gender-specific associations. Thirteen CpGs showed consistent associations between the IoW cohort and the ALSPAC cohort (11 of which were gender-specific).

Conclusion: pre-adolescence DNAm is associated with the change in BMI status from pre- to post-adolescence and such associations are likely to be gender-specific.

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Accepted/In Press date: 28 February 2021
Published date: 25 March 2021
Keywords: Adolescence, BMI status transition, Body mass index (BMI), DNA methylation, Longitudinal, Obesity

Identifiers

Local EPrints ID: 449951
URI: http://eprints.soton.ac.uk/id/eprint/449951
ISSN: 1868-7075
PURE UUID: c79f261c-ba35-47ab-aaee-017ba478c876
ORCID for Faisal I Rezwan: ORCID iD orcid.org/0000-0001-9921-222X
ORCID for John W Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 29 Jun 2021 16:36
Last modified: 17 Jul 2021 01:48

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Contributors

Author: Jiajing Wang
Author: Hongmei Zhang
Author: Faisal I Rezwan ORCID iD
Author: Caroline Relton
Author: S. Hasan Arshad
Author: John W Holloway ORCID iD

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