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A 16384-electrode 1024-channel multimodal CMOS MEA for high-throughput intracellular action potential measurements and impedance spectroscopy in drug-screening applications

A 16384-electrode 1024-channel multimodal CMOS MEA for high-throughput intracellular action potential measurements and impedance spectroscopy in drug-screening applications
A 16384-electrode 1024-channel multimodal CMOS MEA for high-throughput intracellular action potential measurements and impedance spectroscopy in drug-screening applications
Patch clamp is currently the gold standard for studying cell electrophysiology in preclinical drug discovery. Since it is a time-consuming technique, passive and active multielectrode arrays (MEAs) have been introduced for increased throughput in extracellular (ExC) in vitro measurements [1-3]. However, most of these tools cannot capture the essential features of intracellular (InC) action potentials (APs) used for studying drug toxicity. It has been shown that InC access can be achieved by highly localized electroporation [4], which allows low-impedance electrical recording of the InC voltage. While this technique was explored in recent designs [4-5], a tool that enables high-throughput InC measurements is not yet available. Impedance measurement is also used to study cell morphology, adhesion, differentiation and contractility [6]. Recent designs [1-3] already include impedance spectroscopy (IS), but their methods are not fast enough to capture in detail the contractile activity of cardiomyocytes. Since some drugs can inhibit cell beating without affecting the APs [6], ExC/InC recording and impedance measurement are complementary.
464-466
Institute of Electrical and Electronics Engineers Inc.
Lopez, Carolina Mora
b7db9e25-fd8b-4d0e-8e91-2e201640a1eb
Chun, Ho Sung
6ad26c90-a735-46ac-89f1-05ab383a05b9
Berti, Laurent
475e1e9c-5225-4904-91ec-661e2a3aebc0
Wang, Shiwei
97433cb6-7752-4c68-89f8-933f233d8642
Putzeys, Jan
91ca8966-67bd-4b72-82f6-023b78437154
Van den Bulcke, Carl
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Weijers, Jan-Willem
e5d19ec4-eb95-4507-850a-f5bed37b209f
Firrincieli, Andrea
01334896-43a3-463e-9aa5-1cf4d952000b
Reumers, Veerle
0aefdd19-5766-4012-9de8-800d12d60730
Braeken, Dries
9f0912a6-07eb-4f1c-908b-3e8a74ef468e
Van Helleputte, Nick
bad8e4b6-6d24-440f-97c3-e1ddeda371f1
Lopez, Carolina Mora
b7db9e25-fd8b-4d0e-8e91-2e201640a1eb
Chun, Ho Sung
6ad26c90-a735-46ac-89f1-05ab383a05b9
Berti, Laurent
475e1e9c-5225-4904-91ec-661e2a3aebc0
Wang, Shiwei
97433cb6-7752-4c68-89f8-933f233d8642
Putzeys, Jan
91ca8966-67bd-4b72-82f6-023b78437154
Van den Bulcke, Carl
3a6023d6-2b7c-4aa4-89c3-739e96459b02
Weijers, Jan-Willem
e5d19ec4-eb95-4507-850a-f5bed37b209f
Firrincieli, Andrea
01334896-43a3-463e-9aa5-1cf4d952000b
Reumers, Veerle
0aefdd19-5766-4012-9de8-800d12d60730
Braeken, Dries
9f0912a6-07eb-4f1c-908b-3e8a74ef468e
Van Helleputte, Nick
bad8e4b6-6d24-440f-97c3-e1ddeda371f1

Lopez, Carolina Mora, Chun, Ho Sung, Berti, Laurent, Wang, Shiwei, Putzeys, Jan, Van den Bulcke, Carl, Weijers, Jan-Willem, Firrincieli, Andrea, Reumers, Veerle, Braeken, Dries and Van Helleputte, Nick (2018) A 16384-electrode 1024-channel multimodal CMOS MEA for high-throughput intracellular action potential measurements and impedance spectroscopy in drug-screening applications. In 2018 IEEE International Solid - State Circuits Conference - (ISSCC). Institute of Electrical and Electronics Engineers Inc. pp. 464-466 . (doi:10.1109/ISSCC.2018.8310385).

Record type: Conference or Workshop Item (Paper)

Abstract

Patch clamp is currently the gold standard for studying cell electrophysiology in preclinical drug discovery. Since it is a time-consuming technique, passive and active multielectrode arrays (MEAs) have been introduced for increased throughput in extracellular (ExC) in vitro measurements [1-3]. However, most of these tools cannot capture the essential features of intracellular (InC) action potentials (APs) used for studying drug toxicity. It has been shown that InC access can be achieved by highly localized electroporation [4], which allows low-impedance electrical recording of the InC voltage. While this technique was explored in recent designs [4-5], a tool that enables high-throughput InC measurements is not yet available. Impedance measurement is also used to study cell morphology, adhesion, differentiation and contractility [6]. Recent designs [1-3] already include impedance spectroscopy (IS), but their methods are not fast enough to capture in detail the contractile activity of cardiomyocytes. Since some drugs can inhibit cell beating without affecting the APs [6], ExC/InC recording and impedance measurement are complementary.

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More information

e-pub ahead of print date: 12 March 2018
Published date: 2018

Identifiers

Local EPrints ID: 449962
URI: http://eprints.soton.ac.uk/id/eprint/449962
PURE UUID: 6be895d1-3cd4-4f17-a258-a8fceea24413
ORCID for Shiwei Wang: ORCID iD orcid.org/0000-0002-5450-2108

Catalogue record

Date deposited: 29 Jun 2021 17:03
Last modified: 26 Nov 2021 03:23

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Contributors

Author: Carolina Mora Lopez
Author: Ho Sung Chun
Author: Laurent Berti
Author: Shiwei Wang ORCID iD
Author: Jan Putzeys
Author: Carl Van den Bulcke
Author: Jan-Willem Weijers
Author: Andrea Firrincieli
Author: Veerle Reumers
Author: Dries Braeken
Author: Nick Van Helleputte

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