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Acclimation to cyclic hypoxia improves thermal tolerance and copper survival in the caridean shrimp Palaemon varians

Acclimation to cyclic hypoxia improves thermal tolerance and copper survival in the caridean shrimp Palaemon varians
Acclimation to cyclic hypoxia improves thermal tolerance and copper survival in the caridean shrimp Palaemon varians

In response to the continuous variation of environmental parameters, species must be able to adjust their physiology to overcome stressful conditions, a process known as acclimatization. Numerous laboratory studies have been conducted to understand and describe the mechanisms of acclimation to one environmental stressor (e.g. cyclic hypoxia), but currently our understanding of how acclimation to one stressor can change tolerance to a subsequent stressor is limited. Here, in two different experiments, we used the shrimp Palaemon varians to test how, following 28-days acclimation to cyclic hypoxia (mimicking a cyclic hypoxic regime currently found in its natural habitat), critical thermal maximum (CT max) and sensitivity to copper (Cu 2+) exposure (30 mgL −1) changed in comparison to shrimp acclimated to normoxic conditions and then exposed to thermal stress or Cu 2+. Acclimation to cyclic hypoxia improved both CT max (~1 °C higher than controls) and survival to acute Cu 2+ exposure (~30% higher than controls) and induced significant gene expression changes (i.e. up-regulation of heat shock protein 70 – HSP70, hypoxia inducible factor – HIF, phosphoenolpyruvate carboxykinase – PEPCK, glucose 6-P transporter – G6Pt, metallothionein – Mt, and down-regulation of hemocyanin – Hem) in animals acclimated to cyclic hypoxia. Our results demonstrate how acclimation to cyclic hypoxia improved tolerance to subsequent stressors, highlighting the complexity of predicting organismal performance in variable (i.e. where multiple parameters can simultaneously change during the day) environments.

Acclimation to hypoxia, Caridea, Copper survival, Temperature tolerance
1095-6433
Peruzza, Luca
97dfdd24-f9e8-4d76-aee5-67bdd9b92541
Thatje, Sven
f1011fe3-1048-40c0-97c1-e93b796e6533
Hauton, Chris
7706f6ba-4497-42b2-8c6d-00df81676331
Peruzza, Luca
97dfdd24-f9e8-4d76-aee5-67bdd9b92541
Thatje, Sven
f1011fe3-1048-40c0-97c1-e93b796e6533
Hauton, Chris
7706f6ba-4497-42b2-8c6d-00df81676331

Peruzza, Luca, Thatje, Sven and Hauton, Chris (2021) Acclimation to cyclic hypoxia improves thermal tolerance and copper survival in the caridean shrimp Palaemon varians. Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology, 259, [111010]. (doi:10.1016/j.cbpa.2021.111010).

Record type: Article

Abstract

In response to the continuous variation of environmental parameters, species must be able to adjust their physiology to overcome stressful conditions, a process known as acclimatization. Numerous laboratory studies have been conducted to understand and describe the mechanisms of acclimation to one environmental stressor (e.g. cyclic hypoxia), but currently our understanding of how acclimation to one stressor can change tolerance to a subsequent stressor is limited. Here, in two different experiments, we used the shrimp Palaemon varians to test how, following 28-days acclimation to cyclic hypoxia (mimicking a cyclic hypoxic regime currently found in its natural habitat), critical thermal maximum (CT max) and sensitivity to copper (Cu 2+) exposure (30 mgL −1) changed in comparison to shrimp acclimated to normoxic conditions and then exposed to thermal stress or Cu 2+. Acclimation to cyclic hypoxia improved both CT max (~1 °C higher than controls) and survival to acute Cu 2+ exposure (~30% higher than controls) and induced significant gene expression changes (i.e. up-regulation of heat shock protein 70 – HSP70, hypoxia inducible factor – HIF, phosphoenolpyruvate carboxykinase – PEPCK, glucose 6-P transporter – G6Pt, metallothionein – Mt, and down-regulation of hemocyanin – Hem) in animals acclimated to cyclic hypoxia. Our results demonstrate how acclimation to cyclic hypoxia improved tolerance to subsequent stressors, highlighting the complexity of predicting organismal performance in variable (i.e. where multiple parameters can simultaneously change during the day) environments.

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Acclimation to cyclic hypoxia improves thermal tolerance and copper survival - Accepted Manuscript
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More information

Accepted/In Press date: 2 June 2021
e-pub ahead of print date: 5 June 2021
Published date: 1 September 2021
Keywords: Acclimation to hypoxia, Caridea, Copper survival, Temperature tolerance

Identifiers

Local EPrints ID: 450049
URI: http://eprints.soton.ac.uk/id/eprint/450049
ISSN: 1095-6433
PURE UUID: 40608a3b-79cf-4389-9c98-a8bf4467be9e
ORCID for Chris Hauton: ORCID iD orcid.org/0000-0002-2313-4226

Catalogue record

Date deposited: 07 Jul 2021 16:30
Last modified: 26 Nov 2021 02:38

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Contributors

Author: Luca Peruzza
Author: Sven Thatje
Author: Chris Hauton ORCID iD

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