Anti-inflammatory effects of oleic acid and the anthocyanin keracyanin alone and in combination: effects on monocyte and macrophage responses and the NF-kappa B pathway
Anti-inflammatory effects of oleic acid and the anthocyanin keracyanin alone and in combination: effects on monocyte and macrophage responses and the NF-kappa B pathway
Monocyte recruitment and activation of macrophages are essential for homeostasis but are also related to the development and progression of cardiometabolic diseases. The management of inflammation with dietary components has been widely investigated. Two components that may influence inflammation are unsaturated fatty acids such as oleic acid (OA; 18:1cis-9) and antioxidant compounds like anthocyanins. Molecular and metabolic effects of such bioactive compounds are usually investigated in isolation, whereas they may be present in combination in foods or the diet. Considering this, we aimed to analyze the effects of OA and the anthocyanin keracyanin (AC) alone and in combination on toll-like receptor-mediated inflammatory responses in monocytes and macrophages. For this, THP-1-derived macrophages and monocytes were exposed to 3 treatments: OA, AC, or the combination (OAAC) and then stimulated with lipopolysaccharide. Inflammation-related gene expression and protein concentrations of IL-1β, TNF-α, IL-6, MCP-1, and IL-10 were assessed. Also, NFκBp65, IκBα, and PPAR-γ protein expression were determined. OA, AC, and OAAC decreased pNFκBp65, PPARγ, IκBα, TNF-α, IL-1β, IL-6, and MCP-1 and increased IL-10. MCP-1 protein expression was lower with OAAC than with either OA and AC alone. Compared to control, OAAC decreased mRNA for TLR4, IκKα, IκBα, NFκB1, MCP-1, TNF-α, IL-6, and IL-1β more than OA or AC did alone. Also, IL-10 mRNA was increased by OAAC compared with control, OA, and AC. In summary, OA and AC have anti-inflammatory effects individually but their combination (OAAC) exerts a greater effect.
7909-7922
Santamarina, Aline B.
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Pisani, Luciana P.
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Baker, Ella
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Marat, Andreea D. Marat;
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Valenzuela, Carina
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Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
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7 September 2021
Santamarina, Aline B.
fe60edf1-67e9-4fa2-afa8-fd9613bd1011
Pisani, Luciana P.
febe1f97-5000-4f4b-a255-93eb0c6ac62d
Baker, Ella
7cd5b762-d7d7-4584-b9a7-dba555085440
Marat, Andreea D. Marat;
539070a5-8651-4b69-ba34-eb15f1a8ecc0
Valenzuela, Carina
1a12a9b9-6504-4392-90c5-246644b0ad5c
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Santamarina, Aline B., Pisani, Luciana P., Baker, Ella, Marat, Andreea D. Marat;, Valenzuela, Carina, Miles, Elizabeth and Calder, Philip
(2021)
Anti-inflammatory effects of oleic acid and the anthocyanin keracyanin alone and in combination: effects on monocyte and macrophage responses and the NF-kappa B pathway.
Food & Function, 12 (17), .
(doi:10.1039/D1FO01304A).
Abstract
Monocyte recruitment and activation of macrophages are essential for homeostasis but are also related to the development and progression of cardiometabolic diseases. The management of inflammation with dietary components has been widely investigated. Two components that may influence inflammation are unsaturated fatty acids such as oleic acid (OA; 18:1cis-9) and antioxidant compounds like anthocyanins. Molecular and metabolic effects of such bioactive compounds are usually investigated in isolation, whereas they may be present in combination in foods or the diet. Considering this, we aimed to analyze the effects of OA and the anthocyanin keracyanin (AC) alone and in combination on toll-like receptor-mediated inflammatory responses in monocytes and macrophages. For this, THP-1-derived macrophages and monocytes were exposed to 3 treatments: OA, AC, or the combination (OAAC) and then stimulated with lipopolysaccharide. Inflammation-related gene expression and protein concentrations of IL-1β, TNF-α, IL-6, MCP-1, and IL-10 were assessed. Also, NFκBp65, IκBα, and PPAR-γ protein expression were determined. OA, AC, and OAAC decreased pNFκBp65, PPARγ, IκBα, TNF-α, IL-1β, IL-6, and MCP-1 and increased IL-10. MCP-1 protein expression was lower with OAAC than with either OA and AC alone. Compared to control, OAAC decreased mRNA for TLR4, IκKα, IκBα, NFκB1, MCP-1, TNF-α, IL-6, and IL-1β more than OA or AC did alone. Also, IL-10 mRNA was increased by OAAC compared with control, OA, and AC. In summary, OA and AC have anti-inflammatory effects individually but their combination (OAAC) exerts a greater effect.
Text
Food&Function_Accepted version
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More information
Accepted/In Press date: 24 June 2021
e-pub ahead of print date: 25 June 2021
Published date: 7 September 2021
Additional Information:
Funding Information:
The authors acknowledge financial support from Funda?a?o de Amparo ? Pesquisa do Estado de Sa?o Paulo (FAPESP 2018/23623-7; 2015/13875-0). LPP thanks CNPq (Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico) fellowship.
Funding Information:
The authors acknowledge financial support from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2018/ 23623-7; 2015/13875-0). LPP thanks CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) fellowship.
Publisher Copyright:
© The Royal Society of Chemistry.
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Local EPrints ID: 450073
URI: http://eprints.soton.ac.uk/id/eprint/450073
PURE UUID: 6701006a-18e2-4810-ac8c-2bc44eb45d65
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Date deposited: 08 Jul 2021 16:31
Last modified: 17 Mar 2024 06:40
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Contributors
Author:
Aline B. Santamarina
Author:
Luciana P. Pisani
Author:
Ella Baker
Author:
Andreea D. Marat; Marat
Author:
Carina Valenzuela
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