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Anti-inflammatory effects of oleic acid and the anthocyanin keracyanin alone and in combination: effects on monocyte and macrophage responses and the NF-kappa B pathway

Anti-inflammatory effects of oleic acid and the anthocyanin keracyanin alone and in combination: effects on monocyte and macrophage responses and the NF-kappa B pathway
Anti-inflammatory effects of oleic acid and the anthocyanin keracyanin alone and in combination: effects on monocyte and macrophage responses and the NF-kappa B pathway

The recruitment of monocytes and activation of macrophages is essential for homeostasis but is also related to the development and progression of cardiometabolic diseases. The management of inflammation through dietary components has been widely investigated. Two renowned components that may influence inflammation are unsaturated fatty acids such as oleic acid (OA; 18:1cis-9) and antioxidant compounds like anthocyanins. Molecular and metabolic effects of such bioactive compounds are usually investigated in isolation, whereas they may be present in combination in foods or in the diet. Considering this, we aimed to analyse the effects of OA and the anthocyanin keracyanin (AC) alone and in combination on toll-like receptor-mediated inflammatory responses in monocytes and macrophages. For this, THP-1-derived macrophages and monocytes were exposed to 3 treatments: OA, AC, or the combination (OAAC) and then stimulated with lipopolysaccharide. Inflammatory-related gene expression and protein concentrations of IL-1β, TNF-α, IL-6, MCP-1 and IL-10 were assessed. Also, NFκBp65, IκBα and PPAR-γ protein expression were determined. OA, AC and OAAC decreased pNFκBp65, PPARγ, IκBα, TNF-α, IL-1β, IL-6, and MCP-1 and increased IL-10. MCP-1 protein expression was lower with OAAC than with either OA and AC alone. Compared to CTL, OAAC decreased mRNA for TLR4, IκKα, IκBα, NFκB1, MCP-1, TNF-α, IL-6, and IL-1β more than OA or AC did alone. Also, IL-10 mRNA was increased by OAAC compared with CTL, OA and AC. In summary, OA and AC have anti-inflammatory effects individually but their combination (OAAC) exerts a greater effect.
7909-7922
Santamarina, Aline B.
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Pisani, Luciana P.
febe1f97-5000-4f4b-a255-93eb0c6ac62d
Baker, Ella
7cd5b762-d7d7-4584-b9a7-dba555085440
Marat, Andreea D. Marat;
539070a5-8651-4b69-ba34-eb15f1a8ecc0
Valenzuela, Carina
1a12a9b9-6504-4392-90c5-246644b0ad5c
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Santamarina, Aline B.
fe60edf1-67e9-4fa2-afa8-fd9613bd1011
Pisani, Luciana P.
febe1f97-5000-4f4b-a255-93eb0c6ac62d
Baker, Ella
7cd5b762-d7d7-4584-b9a7-dba555085440
Marat, Andreea D. Marat;
539070a5-8651-4b69-ba34-eb15f1a8ecc0
Valenzuela, Carina
1a12a9b9-6504-4392-90c5-246644b0ad5c
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6

Santamarina, Aline B., Pisani, Luciana P., Baker, Ella, Marat, Andreea D. Marat;, Valenzuela, Carina, Miles, Elizabeth and Calder, Philip (2021) Anti-inflammatory effects of oleic acid and the anthocyanin keracyanin alone and in combination: effects on monocyte and macrophage responses and the NF-kappa B pathway. Food & Function, 12 (17), 7909-7922. (doi:10.1039/D1FO01304A).

Record type: Article

Abstract


The recruitment of monocytes and activation of macrophages is essential for homeostasis but is also related to the development and progression of cardiometabolic diseases. The management of inflammation through dietary components has been widely investigated. Two renowned components that may influence inflammation are unsaturated fatty acids such as oleic acid (OA; 18:1cis-9) and antioxidant compounds like anthocyanins. Molecular and metabolic effects of such bioactive compounds are usually investigated in isolation, whereas they may be present in combination in foods or in the diet. Considering this, we aimed to analyse the effects of OA and the anthocyanin keracyanin (AC) alone and in combination on toll-like receptor-mediated inflammatory responses in monocytes and macrophages. For this, THP-1-derived macrophages and monocytes were exposed to 3 treatments: OA, AC, or the combination (OAAC) and then stimulated with lipopolysaccharide. Inflammatory-related gene expression and protein concentrations of IL-1β, TNF-α, IL-6, MCP-1 and IL-10 were assessed. Also, NFκBp65, IκBα and PPAR-γ protein expression were determined. OA, AC and OAAC decreased pNFκBp65, PPARγ, IκBα, TNF-α, IL-1β, IL-6, and MCP-1 and increased IL-10. MCP-1 protein expression was lower with OAAC than with either OA and AC alone. Compared to CTL, OAAC decreased mRNA for TLR4, IκKα, IκBα, NFκB1, MCP-1, TNF-α, IL-6, and IL-1β more than OA or AC did alone. Also, IL-10 mRNA was increased by OAAC compared with CTL, OA and AC. In summary, OA and AC have anti-inflammatory effects individually but their combination (OAAC) exerts a greater effect.

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Accepted/In Press date: 24 June 2021
e-pub ahead of print date: 25 June 2021
Published date: 25 June 2021

Identifiers

Local EPrints ID: 450073
URI: http://eprints.soton.ac.uk/id/eprint/450073
PURE UUID: 6701006a-18e2-4810-ac8c-2bc44eb45d65
ORCID for Elizabeth Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for Philip Calder: ORCID iD orcid.org/0000-0002-6038-710X

Catalogue record

Date deposited: 08 Jul 2021 16:31
Last modified: 22 Nov 2021 02:39

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Contributors

Author: Aline B. Santamarina
Author: Luciana P. Pisani
Author: Ella Baker
Author: Andreea D. Marat; Marat
Author: Carina Valenzuela
Author: Elizabeth Miles ORCID iD
Author: Philip Calder ORCID iD

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