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Dual Dean entrainment with volume ratio modulation for efficient droplet co-encapsulation: extreme single-cell indexing

Dual Dean entrainment with volume ratio modulation for efficient droplet co-encapsulation: extreme single-cell indexing
Dual Dean entrainment with volume ratio modulation for efficient droplet co-encapsulation: extreme single-cell indexing
The future of single cell diversity screens involves ever-larger sample sizes, dictating the need for higher throughput methods with low analytical noise to accurately describe the nature of the cellular system. Current approaches are limited by the Poisson statistic, requiring dilute cell suspensions and associated losses in throughput. In this contribution, we apply Dean entrainment to both cell and bead inputs, defining different volume packets to effect efficient co-encapsulation. Volume ratio scaling was explored to identify optimal conditions. This enabled the co-encapsulation of single cells with reporter beads at rates of ∼1 million cells per hour, while increasing assay signal-to-noise with cell multiplet rates of ∼2.5% and capturing ∼70% of cells. The method, called Pirouette coupling, extends our capacity to investigate biological systems.
1473-0197
3378-3386
Harrington, Jack
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Esteban, Luis Blay
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Butement, Jonathan
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Vallejo, Andres F.
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Lane, Simon I.R.
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Sheth, Bhavwanti
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Jongen, Maaike S.A.
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Parker, Rachel
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Stumpf, Patrick S.
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Smith, Rosanna C.G.
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MacArthur, Ben D.
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Rose-Zerilli, Matthew J.J.
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Polak, Marta E.
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Underwood, Tim
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West, Jonathan
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Harrington, Jack
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Esteban, Luis Blay
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Butement, Jonathan
581ce321-f1af-4a2f-870a-9d8d45133586
Vallejo, Andres F.
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Lane, Simon I.R.
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Sheth, Bhavwanti
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Jongen, Maaike S.A.
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Parker, Rachel
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Stumpf, Patrick S.
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Smith, Rosanna C.G.
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MacArthur, Ben D.
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Rose-Zerilli, Matthew J.J.
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Polak, Marta E.
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Underwood, Tim
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West, Jonathan
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Harrington, Jack, Esteban, Luis Blay, Butement, Jonathan, Vallejo, Andres F., Lane, Simon I.R., Sheth, Bhavwanti, Jongen, Maaike S.A., Parker, Rachel, Stumpf, Patrick S., Smith, Rosanna C.G., MacArthur, Ben D., Rose-Zerilli, Matthew J.J., Polak, Marta E., Underwood, Tim and West, Jonathan (2021) Dual Dean entrainment with volume ratio modulation for efficient droplet co-encapsulation: extreme single-cell indexing. Lab on a Chip, 17 (21), 3378-3386. (doi:10.1101/2021.04.08.439026).

Record type: Article

Abstract

The future of single cell diversity screens involves ever-larger sample sizes, dictating the need for higher throughput methods with low analytical noise to accurately describe the nature of the cellular system. Current approaches are limited by the Poisson statistic, requiring dilute cell suspensions and associated losses in throughput. In this contribution, we apply Dean entrainment to both cell and bead inputs, defining different volume packets to effect efficient co-encapsulation. Volume ratio scaling was explored to identify optimal conditions. This enabled the co-encapsulation of single cells with reporter beads at rates of ∼1 million cells per hour, while increasing assay signal-to-noise with cell multiplet rates of ∼2.5% and capturing ∼70% of cells. The method, called Pirouette coupling, extends our capacity to investigate biological systems.

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2021.04.08.439026v1.full - Author's Original
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Harrington_PirouetteSeq_LOC2021 - Accepted Manuscript
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More information

Submitted date: 6 April 2021
Accepted/In Press date: 21 June 2021
e-pub ahead of print date: 30 June 2021

Identifiers

Local EPrints ID: 450486
URI: http://eprints.soton.ac.uk/id/eprint/450486
ISSN: 1473-0197
PURE UUID: 9a20e15f-f2ee-4ac7-8257-de2233ff28a7
ORCID for Andres F. Vallejo: ORCID iD orcid.org/0000-0002-4688-0598
ORCID for Patrick S. Stumpf: ORCID iD orcid.org/0000-0003-0862-0290
ORCID for Tim Underwood: ORCID iD orcid.org/0000-0001-9455-2188
ORCID for Jonathan West: ORCID iD orcid.org/0000-0002-5709-6790

Catalogue record

Date deposited: 29 Jul 2021 16:32
Last modified: 25 Jul 2024 01:51

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Contributors

Author: Jack Harrington
Author: Luis Blay Esteban
Author: Jonathan Butement
Author: Andres F. Vallejo ORCID iD
Author: Simon I.R. Lane
Author: Bhavwanti Sheth
Author: Maaike S.A. Jongen
Author: Rachel Parker
Author: Patrick S. Stumpf ORCID iD
Author: Rosanna C.G. Smith
Author: Ben D. MacArthur
Author: Matthew J.J. Rose-Zerilli
Author: Marta E. Polak
Author: Tim Underwood ORCID iD
Author: Jonathan West ORCID iD

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