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Maternal intermittent fasting during pregnancy induces fetal growth restriction and down-regulated placental system A amino acid transport in the rat

Maternal intermittent fasting during pregnancy induces fetal growth restriction and down-regulated placental system A amino acid transport in the rat
Maternal intermittent fasting during pregnancy induces fetal growth restriction and down-regulated placental system A amino acid transport in the rat

During Ramadan, many pregnant Muslim women fast between dawn and sunset. Although the impacts of prolonged maternal intermittent fasting (IF) on fetal growth and placental function are under-researched, reported effects include reduced placental weight and birth weight. In the present study, pregnant Wistar rats were used to model repeated cycles of IF on fetal development and placental function and to examine sex-specific effects. In the IF group, food was withdrawn daily from 17:00 to 09:00 over 21 days of gestation, while the control group received food ad libitum. Both groups had free water access. IF dams consumed less food, had significantly reduced weight compared with controls, with reduced plasma glucose and amino acids. Both fetal sexes were significantly lighter in the IF group with reduced fetal plasma amino acids. Placental weights and morphology were unchanged. The profile of placental metabolites was altered in the IF group with sex-specific responses evident. Transplacental flux of 14C-methylaminoisobutyric acid ( 14C-MeAIB), a system A amino acid transporter substrate, was significantly reduced in both fetal sexes in the IF group. Sodium-dependent 14C-MeAIB uptake into isolated placental plasma membrane vesicles was unchanged. The gene expression of system A transporter Slc38a1, Slc38a2 and Slc38a4 was up-regulated in IF male placentas only. No changes were observed in placental SNAT1 and SNAT2 protein expression. Maternal IF results in detrimental impacts on maternal physiology and fetal development with changes in the placental and fetal metabolite profiles. Reduced placental system A transporter activity may be responsible for fetal growth restriction in both sexes.

0143-5221
1445-1466
Alkhalefah, A.
681c7475-9f68-43e5-8adf-b0cc9f0fafba
Dunn, W. B.
49393b65-e019-4231-b200-937766ec43de
Allwood, J. W.
1b89228a-556f-44b9-9a15-fc5196bffd8a
Parry, K. L.
7180a304-dd45-4d0f-aed3-75b52d62a786
Houghton, F. D.
53946041-127e-45a8-9edb-bf4b3c23005f
Ashton, N.
f29b4588-3cca-49b7-97ed-5601db218ddf
Glazier, J. D.
56865fc7-2ffa-47a8-8527-ef785c47f3e0
Alkhalefah, A.
681c7475-9f68-43e5-8adf-b0cc9f0fafba
Dunn, W. B.
49393b65-e019-4231-b200-937766ec43de
Allwood, J. W.
1b89228a-556f-44b9-9a15-fc5196bffd8a
Parry, K. L.
7180a304-dd45-4d0f-aed3-75b52d62a786
Houghton, F. D.
53946041-127e-45a8-9edb-bf4b3c23005f
Ashton, N.
f29b4588-3cca-49b7-97ed-5601db218ddf
Glazier, J. D.
56865fc7-2ffa-47a8-8527-ef785c47f3e0

Alkhalefah, A., Dunn, W. B., Allwood, J. W., Parry, K. L., Houghton, F. D., Ashton, N. and Glazier, J. D. (2021) Maternal intermittent fasting during pregnancy induces fetal growth restriction and down-regulated placental system A amino acid transport in the rat. Clinical Science, 135 (11), 1445-1466. (doi:10.1042/CS20210137).

Record type: Article

Abstract

During Ramadan, many pregnant Muslim women fast between dawn and sunset. Although the impacts of prolonged maternal intermittent fasting (IF) on fetal growth and placental function are under-researched, reported effects include reduced placental weight and birth weight. In the present study, pregnant Wistar rats were used to model repeated cycles of IF on fetal development and placental function and to examine sex-specific effects. In the IF group, food was withdrawn daily from 17:00 to 09:00 over 21 days of gestation, while the control group received food ad libitum. Both groups had free water access. IF dams consumed less food, had significantly reduced weight compared with controls, with reduced plasma glucose and amino acids. Both fetal sexes were significantly lighter in the IF group with reduced fetal plasma amino acids. Placental weights and morphology were unchanged. The profile of placental metabolites was altered in the IF group with sex-specific responses evident. Transplacental flux of 14C-methylaminoisobutyric acid ( 14C-MeAIB), a system A amino acid transporter substrate, was significantly reduced in both fetal sexes in the IF group. Sodium-dependent 14C-MeAIB uptake into isolated placental plasma membrane vesicles was unchanged. The gene expression of system A transporter Slc38a1, Slc38a2 and Slc38a4 was up-regulated in IF male placentas only. No changes were observed in placental SNAT1 and SNAT2 protein expression. Maternal IF results in detrimental impacts on maternal physiology and fetal development with changes in the placental and fetal metabolite profiles. Reduced placental system A transporter activity may be responsible for fetal growth restriction in both sexes.

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Alkhalefah et al. accepted full article for publication - Accepted Manuscript
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Accepted/In Press date: 19 May 2021
e-pub ahead of print date: 11 June 2021
Additional Information: Funding Information: A.A. was supported by a scholarship from the Government of Kuwait. Publisher Copyright: © 2021 Portland Press Ltd. All rights reserved. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

Identifiers

Local EPrints ID: 450630
URI: http://eprints.soton.ac.uk/id/eprint/450630
ISSN: 0143-5221
PURE UUID: ae45eb6b-a485-44c6-b174-673304c159f1
ORCID for F. D. Houghton: ORCID iD orcid.org/0000-0002-5167-1694

Catalogue record

Date deposited: 05 Aug 2021 16:31
Last modified: 06 Aug 2021 01:38

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Contributors

Author: A. Alkhalefah
Author: W. B. Dunn
Author: J. W. Allwood
Author: K. L. Parry
Author: F. D. Houghton ORCID iD
Author: N. Ashton
Author: J. D. Glazier

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