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Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins

Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins
Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins

Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods. Most glycans form [M – H] - or [M + adduct] - ions but larger ones (above approx. m/z 2000) typically form doubly charged ions. Differences have been reported between the fragmentation of singly and doubly charged ions but a detailed comparison does not appear to have been reported. In addition to [M + adduct] - ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [M n + (H 2PO 4) n] n - have been observed in mixtures of N-glycans released from viral and other glycoproteins. This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans. Graphical abstract: [Figure not available: see fulltext.].

Double charge, Fragmentation, N-Glycans, Negative ion, Triple charge
1618-2642
7277-7294
Harvey, David J
8bb24417-3852-4b1f-827b-0d5d2c176744
Struwe, Weston B
16a348b1-3921-4a2d-b5fb-d341fccea65f
Behrens, Anna-Janina
ed584c40-79cb-4de9-bb9c-bd68c71d6a68
Vasiljevic, Snezana
17e075b4-520d-4b9b-a4a7-08ac394ae5e1
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Harvey, David J
8bb24417-3852-4b1f-827b-0d5d2c176744
Struwe, Weston B
16a348b1-3921-4a2d-b5fb-d341fccea65f
Behrens, Anna-Janina
ed584c40-79cb-4de9-bb9c-bd68c71d6a68
Vasiljevic, Snezana
17e075b4-520d-4b9b-a4a7-08ac394ae5e1
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9

Harvey, David J, Struwe, Weston B, Behrens, Anna-Janina, Vasiljevic, Snezana and Crispin, Max (2021) Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins. Analytical and Bioanalytical Chemistry, 413 (29), 7277-7294. (doi:10.1007/s00216-021-03480-8).

Record type: Article

Abstract

Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods. Most glycans form [M – H] - or [M + adduct] - ions but larger ones (above approx. m/z 2000) typically form doubly charged ions. Differences have been reported between the fragmentation of singly and doubly charged ions but a detailed comparison does not appear to have been reported. In addition to [M + adduct] - ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [M n + (H 2PO 4) n] n - have been observed in mixtures of N-glycans released from viral and other glycoproteins. This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans. Graphical abstract: [Figure not available: see fulltext.].

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More information

Accepted/In Press date: 2021
e-pub ahead of print date: 3 August 2021
Published date: December 2021
Keywords: Double charge, Fragmentation, N-Glycans, Negative ion, Triple charge

Identifiers

Local EPrints ID: 450935
URI: http://eprints.soton.ac.uk/id/eprint/450935
ISSN: 1618-2642
PURE UUID: 78802485-cac9-4195-87b7-2e5810265c14
ORCID for Max Crispin: ORCID iD orcid.org/0000-0002-1072-2694

Catalogue record

Date deposited: 24 Aug 2021 17:02
Last modified: 17 Mar 2024 03:47

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Contributors

Author: David J Harvey
Author: Weston B Struwe
Author: Anna-Janina Behrens
Author: Snezana Vasiljevic
Author: Max Crispin ORCID iD

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