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Structure and biosynthesis of glycoprotein carbohydrates

Structure and biosynthesis of glycoprotein carbohydrates
Structure and biosynthesis of glycoprotein carbohydrates

In eukaryotes, the majority of cell surface and secreted proteins are covalently modified with carbohydrates. This type of posttranslational modification, glycosylation, is inherently complex exhibiting extensive chemical and conformational heterogeneity. Despite this complexity, there are common structural and biosynthetic principles. Here, we present an introduction to the different hierarchies of carbohydrate structure from stereochemistry of monosaccharides and their linkages to the structural diversity of complex mammalianglycosylation. These structures are discussed in the context of the glycan biosynthetic pathways, with an emphasis on N-linked glycosylation, and we outline the nomenclature with which these complex structures can be described. We describe the biosynthesis and crystal structures of antibodies and their glycans to illustrate the conformational properties of glycoprotein carbohydrates and how therapeutic antibodies are being developed by modulating these glycans.

51-68
Elsevier
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Scanlan, Christopher N.
04dd1b57-b6fc-414c-8595-08310dbb3d32
Bowden, Thomas A.
4b17a588-ac01-4112-807a-8b99a6c20d0f
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Scanlan, Christopher N.
04dd1b57-b6fc-414c-8595-08310dbb3d32
Bowden, Thomas A.
4b17a588-ac01-4112-807a-8b99a6c20d0f

Crispin, Max, Scanlan, Christopher N. and Bowden, Thomas A. (2019) Structure and biosynthesis of glycoprotein carbohydrates. In, Comprehensive Biotechnology (3rd Edition). Elsevier, pp. 51-68. (doi:10.1016/B978-0-444-64046-8.00007-0).

Record type: Book Section

Abstract

In eukaryotes, the majority of cell surface and secreted proteins are covalently modified with carbohydrates. This type of posttranslational modification, glycosylation, is inherently complex exhibiting extensive chemical and conformational heterogeneity. Despite this complexity, there are common structural and biosynthetic principles. Here, we present an introduction to the different hierarchies of carbohydrate structure from stereochemistry of monosaccharides and their linkages to the structural diversity of complex mammalianglycosylation. These structures are discussed in the context of the glycan biosynthetic pathways, with an emphasis on N-linked glycosylation, and we outline the nomenclature with which these complex structures can be described. We describe the biosynthesis and crystal structures of antibodies and their glycans to illustrate the conformational properties of glycoprotein carbohydrates and how therapeutic antibodies are being developed by modulating these glycans.

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More information

Published date: 1 January 2019
Additional Information: Publisher Copyright: © 2019 Elsevier B.V. All rights reserved. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

Identifiers

Local EPrints ID: 451170
URI: http://eprints.soton.ac.uk/id/eprint/451170
PURE UUID: e4cf3ecf-3dd5-4528-9c5b-897a36c10c0e
ORCID for Max Crispin: ORCID iD orcid.org/0000-0002-1072-2694

Catalogue record

Date deposited: 14 Sep 2021 15:31
Last modified: 15 Sep 2021 02:13

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Contributors

Author: Max Crispin ORCID iD
Author: Christopher N. Scanlan
Author: Thomas A. Bowden

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