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A co-culture nanofibre scaffold model of neural cell degeneration in relevance to Parkinson’s disease

A co-culture nanofibre scaffold model of neural cell degeneration in relevance to Parkinson’s disease
A co-culture nanofibre scaffold model of neural cell degeneration in relevance to Parkinson’s disease
Current therapeutic strategies for Parkinson’s disease (PD) aim to delay progression or replace damaged neurons by restoring the original neuronal structures. The poor regenerative capacity of neural tissue highlights the need for the development of cellular environments to model the pathogenesis of PD. In the current work, we have characterised the growth, survival and response to PD mimetics of human SH-SY5Y neuroblastoma and U-87MG glioblastoma cell lines cultured on polyacrylonitrile (PAN) and Jeffamine® doped polyacrylonitrile (PJ) nano-scaffolds. Differentiation induced by a range of agents was evaluated by immunoassays of neural protein biomarkers. PAN and PJ nanofibre scaffolds provided suitable three-dimensional (3D) environment to support the growth, differentiation and network formation of dopaminergic neuron- and astrocyte-like cell populations, respectively. The scaffolds selectively supported the survival and differentiation of both cell populations with prolonged neuronal survival when exposed to PD mimetics in the presence of astrocytes in a co-culture model. Such 3D nanoscaffold-based assays could aid our understanding of the molecular basis of PD mimetic-induced Parkinsonism and the discovery of neuroprotective agents.
2045-2322
Chemmarappally, Joseph
0d6cada6-6bb5-48e9-af93-34b93e935c84
Pegram, Henry
15f5ce11-91e4-490b-98e5-073cf93e83eb
Abeywickrama, Neranga
5852e9b5-0b51-448d-8016-de49914c9ca3
Fornari, Enzo
60ca1daf-f86f-4f67-89d3-ee6acc9cd74b
Hargreaves, Alan
8eaffc1d-13bf-4df3-9fed-a0fd660e6a8f
De Girolamo, Luigi
c8fedb87-1aab-44e7-a493-beef4a2e63c0
Stevens, Bob
63cee635-da1b-4834-9579-d903e4d4713f
Chemmarappally, Joseph
0d6cada6-6bb5-48e9-af93-34b93e935c84
Pegram, Henry
15f5ce11-91e4-490b-98e5-073cf93e83eb
Abeywickrama, Neranga
5852e9b5-0b51-448d-8016-de49914c9ca3
Fornari, Enzo
60ca1daf-f86f-4f67-89d3-ee6acc9cd74b
Hargreaves, Alan
8eaffc1d-13bf-4df3-9fed-a0fd660e6a8f
De Girolamo, Luigi
c8fedb87-1aab-44e7-a493-beef4a2e63c0
Stevens, Bob
63cee635-da1b-4834-9579-d903e4d4713f

Chemmarappally, Joseph, Pegram, Henry, Abeywickrama, Neranga, Fornari, Enzo, Hargreaves, Alan, De Girolamo, Luigi and Stevens, Bob (2020) A co-culture nanofibre scaffold model of neural cell degeneration in relevance to Parkinson’s disease. Scientific Reports, 10 (1), [2767]. (doi:10.1038/s41598-020-59310-x).

Record type: Article

Abstract

Current therapeutic strategies for Parkinson’s disease (PD) aim to delay progression or replace damaged neurons by restoring the original neuronal structures. The poor regenerative capacity of neural tissue highlights the need for the development of cellular environments to model the pathogenesis of PD. In the current work, we have characterised the growth, survival and response to PD mimetics of human SH-SY5Y neuroblastoma and U-87MG glioblastoma cell lines cultured on polyacrylonitrile (PAN) and Jeffamine® doped polyacrylonitrile (PJ) nano-scaffolds. Differentiation induced by a range of agents was evaluated by immunoassays of neural protein biomarkers. PAN and PJ nanofibre scaffolds provided suitable three-dimensional (3D) environment to support the growth, differentiation and network formation of dopaminergic neuron- and astrocyte-like cell populations, respectively. The scaffolds selectively supported the survival and differentiation of both cell populations with prolonged neuronal survival when exposed to PD mimetics in the presence of astrocytes in a co-culture model. Such 3D nanoscaffold-based assays could aid our understanding of the molecular basis of PD mimetic-induced Parkinsonism and the discovery of neuroprotective agents.

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More information

Accepted/In Press date: 20 January 2020
Published date: 17 February 2020

Identifiers

Local EPrints ID: 451231
URI: http://eprints.soton.ac.uk/id/eprint/451231
ISSN: 2045-2322
PURE UUID: 490cbc74-a273-44ed-894a-bcf1cf9fe1d5

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Date deposited: 14 Sep 2021 20:15
Last modified: 27 Apr 2022 11:39

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Contributors

Author: Joseph Chemmarappally
Author: Henry Pegram
Author: Neranga Abeywickrama
Author: Enzo Fornari
Author: Alan Hargreaves
Author: Luigi De Girolamo
Author: Bob Stevens

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