Effects of calcium on light-activated GTP-binding proteins in squid photoreceptor membranes
Effects of calcium on light-activated GTP-binding proteins in squid photoreceptor membranes
1. At least two distinct G-proteins are activated by light in squid photoreceptor membranes, a 45,000 mol. wt cholera toxin substrate and a 40,000 mol. wt pertussis toxin substrate. 2. The light-stimulated GTPase activity is partially inhibited by pretreatment with either toxin and abolished by treatment with both toxins. 3. At 24 degrees C, a free calcium ion concentration of 1 microM inhibits GTPase activity of both toxin substrates and ADP ribosylation by pertussis toxin. 4. This calcium sensitivity of squid G-proteins may be important in interpretation of experimental results on the phosphoinositide or other signalling pathways in squid visual transduction.
215-221
Baverstock, Jenny
82f3fd4c-2b09-4c0d-8485-15afbc53be59
Saibil, Helen
c122572d-9b54-44b8-b736-dc01ee9dcd12
Fyles, Julie
975ce98f-3bd0-4a48-bf7c-a81f10f25588
Bayer, Kristen
6067a273-f329-4b2b-9b78-a17de5ebaacf
30 June 1991
Baverstock, Jenny
82f3fd4c-2b09-4c0d-8485-15afbc53be59
Saibil, Helen
c122572d-9b54-44b8-b736-dc01ee9dcd12
Fyles, Julie
975ce98f-3bd0-4a48-bf7c-a81f10f25588
Bayer, Kristen
6067a273-f329-4b2b-9b78-a17de5ebaacf
Baverstock, Jenny, Saibil, Helen, Fyles, Julie and Bayer, Kristen
(1991)
Effects of calcium on light-activated GTP-binding proteins in squid photoreceptor membranes.
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 98 (2-3), .
(doi:10.1016/0305-0491(91)90169-e).
Abstract
1. At least two distinct G-proteins are activated by light in squid photoreceptor membranes, a 45,000 mol. wt cholera toxin substrate and a 40,000 mol. wt pertussis toxin substrate. 2. The light-stimulated GTPase activity is partially inhibited by pretreatment with either toxin and abolished by treatment with both toxins. 3. At 24 degrees C, a free calcium ion concentration of 1 microM inhibits GTPase activity of both toxin substrates and ADP ribosylation by pertussis toxin. 4. This calcium sensitivity of squid G-proteins may be important in interpretation of experimental results on the phosphoinositide or other signalling pathways in squid visual transduction.
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Published date: 30 June 1991
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Local EPrints ID: 451885
URI: http://eprints.soton.ac.uk/id/eprint/451885
ISSN: 1096-4959
PURE UUID: 57efd78a-fb17-4798-952f-1a138b8e28c1
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Date deposited: 02 Nov 2021 17:43
Last modified: 17 Mar 2024 02:58
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Author:
Helen Saibil
Author:
Julie Fyles
Author:
Kristen Bayer
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