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Effect of the C-terminal proline repeats on ordered packing of squid rhodopsin and its mobility in membranes

Effect of the C-terminal proline repeats on ordered packing of squid rhodopsin and its mobility in membranes
Effect of the C-terminal proline repeats on ordered packing of squid rhodopsin and its mobility in membranes
Negative stain electron microscopy and saturation transfer electron spin resonance spectroscopy have been used to compare the lattice ordering and in-plane membrane mobility of full-length and C-terminally cleaved squid rhodopsin. The C-terminus of squid rhodopsin contains a negatively charged region followed by 9-10 repeats of a proline-rich sequence, not found in rhodopsins other than those of cephalopod invertebrates, but similar proline repeats are found in other, unrelated membrane proteins. We find that the proline repeats cluster the rhodopsins into small groups, interfering with two-dimensional crystallization and maintaining their mobility in the membrane.
0014-5793
45-49
Baverstock, Jenny
82f3fd4c-2b09-4c0d-8485-15afbc53be59
Venien-Bryan, Christina
2605ed6f-ffad-4a97-8384-d2c6dc5ae09c
Saibil, Helen
a103f3c3-c4d8-4438-b977-cf146603a94a
Davies, Anthony
c599bb52-a4d3-4294-8e19-de4c9497ed84
Anthony Watts
Keith Langmack
Derek Marsh
Baverstock, Jenny
82f3fd4c-2b09-4c0d-8485-15afbc53be59
Venien-Bryan, Christina
2605ed6f-ffad-4a97-8384-d2c6dc5ae09c
Saibil, Helen
a103f3c3-c4d8-4438-b977-cf146603a94a
Davies, Anthony
c599bb52-a4d3-4294-8e19-de4c9497ed84

Baverstock, Jenny , Anthony Watts, Keith Langmack and Derek Marsh (1995) Effect of the C-terminal proline repeats on ordered packing of squid rhodopsin and its mobility in membranes. FEBS Letters, 359, 45-49. (doi:10.1016/0014-5793(94)01449-b).

Record type: Article

Abstract

Negative stain electron microscopy and saturation transfer electron spin resonance spectroscopy have been used to compare the lattice ordering and in-plane membrane mobility of full-length and C-terminally cleaved squid rhodopsin. The C-terminus of squid rhodopsin contains a negatively charged region followed by 9-10 repeats of a proline-rich sequence, not found in rhodopsins other than those of cephalopod invertebrates, but similar proline repeats are found in other, unrelated membrane proteins. We find that the proline repeats cluster the rhodopsins into small groups, interfering with two-dimensional crystallization and maintaining their mobility in the membrane.

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Accepted/In Press date: 6 February 1995
Published date: 6 February 1995

Identifiers

Local EPrints ID: 451887
URI: http://eprints.soton.ac.uk/id/eprint/451887
DOI: doi:10.1016/0014-5793(94)01449-b
ISSN: 0014-5793
PURE UUID: 5e2fb14d-2054-4337-b6e7-ad536717196e
ORCID for Jenny Baverstock: ORCID iD orcid.org/0000-0003-1777-2894

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Date deposited: 02 Nov 2021 17:43
Last modified: 17 Mar 2024 02:58

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Author: Jenny Baverstock ORCID iD
Author: Christina Venien-Bryan
Author: Helen Saibil
Author: Anthony Davies
Corporate Author: Anthony Watts
Corporate Author: Keith Langmack
Corporate Author: Derek Marsh

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