Readme for data used in the article 'The effect of haematocrit on the mid-infrared refractive index of blood plasma,' published by Biosensors. This readme was created in November 2021.

Dataset DOI: https://doi.org/10.5258/SOTON/D1621
Article DOI: https://doi.org/10.3390/bios11110417

This data is used in the article 'The effect of haematocrit on the mid-infrared refractive index of blood plasma,' published by Biosensors.  The data contained in data.xlsx are those used to plot the figures in the article.

Measurement data were collected by ATR-FTIR spectroscopy at the University of Southampton during December 2019.  Full methodological details can be found in the article.

The XY data for each figure are contained in separate worksheets within data.xlsx.  Each dataset is labelled with its name and unit.  For plots with several spectral traces with respect to wavenumber, each trace is sampled at identical wavenumbers so wavenumber is only listed once.

Briefly, each figure shows:
 
Figure 1: Absorbance spectra of (a) DI water, plasma and whole blood with haematocrit in the range 20–70%, (b) plasma and whole blood with haematocrit in the range 20–70% over a more limited frequency range (1370–1570 cm−1), and (c) absorbance at 1541 cm−1 with respect to haematocrit.
 
Figure 2: Empirical effective penetration depth deff calculated from the measured absorbance and literature k values of water.  The dashed trace at wavenumbers > 3700 cm^-1 show the region where deff has been calculated from a ratio where both quantities are approximately equal to zero so cannot be relied upon.
 
Figure 3: Imaginary part of refractive index spectra k for  water and whole blood with haematocrit in the range 20 – 70%.
 
Figure 4: Real part of refractive index spectra n for water and whole blood with haematocrit in the range 20 – 70%.
 
Figure 5: Error in (a) real and (c) imaginary parts of plasma refractive index due to haematocrit in the range 20 – 70%.  (b) shows the maximum error in n, which occurs at 1560 cm^-1, with respect to haematocrit; (d) shows the corresponding behaviour for k, which occurs at 1541 cm^-1.

The data may be reused under Creative Common Attribution v4.0.

Authors
David J Rowe (1), Daniel R Owens (2, 3), Suzanne L Parker (4), Saul N Faust (2, 3), James S Wilkinson (1) and Goran Z Mashanovich (1)

Affiliations
(1) Zepler Institute for Photonics and Nanoelectronics
University of Southampton
Highfield
Southampton SO17 1BJ
UK

(2) Faculty of Medicine and Institute for Life Sciences
University of Southampton 
Tremona Road
Southampton SO16 6YD
UK

(3)  NIHR Southampton Clinical Research Facility and Biomedical Research Centre
University Hospital Southampton NHS Foundation Trust
Tremona Road
Southampton SO16 6YD
UK

(4) University of Queensland Centre for Clinical Research
The University of Queensland
Herston QLD 4029
Australia