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The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood

The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood
The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood
Recent advances suggest that miniaturised mid-infrared (MIR) devices could replace more time-consuming, laboratory-based techniques for clinical diagnostics. This work uses Fourier transform infrared spectroscopy to show that the MIR complex refractive index of whole blood varies across a range of haematocrit. This indicates that the use of an evanescent measurement is not sufficient to optically exclude the cellular content of blood in the MIR, as previously assumed. Here, spectral refractive index data is presented in two ways. First, it is given as whole blood with varying haematocrit. Second, it is given as the percentage error that haematocrit introduces to plasma. The maximum error in the effective plasma refractive index due to the haematocrit of healthy adults was 0.25% for the real part n and 11% for the imaginary part k. This implies that calibration measurements of haematocrit can be used to account for errors introduced by the cellular content, enabling plasma spectra and analyte concentrations to be indirectly calculated from a whole blood sample. This methodological advance is of clinical importance as plasma concentration of analytes such as drugs can be determined using MIR without the preprocessing of whole blood.
Bioanalytical validation, Biofluid analysis, Mid-infrared spectroscopy, Point-of-care sensing
2079-6374
Rowe, David
a0e0fe82-5e29-42b8-b370-5236a722f015
Owens, Daniel
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Parker, Suzanne
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Faust, Saul
f97df780-9f9b-418e-b349-7adf63e150c1
Wilkinson, James S
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Mashanovich, Goran
c806e262-af80-4836-b96f-319425060051
Rowe, David
a0e0fe82-5e29-42b8-b370-5236a722f015
Owens, Daniel
14ffebb7-6124-4f61-89c1-907248d111b6
Parker, Suzanne
32cbbbb4-6d5f-4135-9f46-f6e3145b21a7
Faust, Saul
f97df780-9f9b-418e-b349-7adf63e150c1
Wilkinson, James S
73483cf3-d9f2-4688-9b09-1c84257884ca
Mashanovich, Goran
c806e262-af80-4836-b96f-319425060051

Rowe, David, Owens, Daniel, Parker, Suzanne, Faust, Saul, Wilkinson, James S and Mashanovich, Goran (2021) The Effect of Haematocrit on Measurement of the Mid-Infrared Refractive Index of Plasma in Whole Blood. Biosensors, 11 (11), [417]. (doi:10.3390/bios11110417).

Record type: Article

Abstract

Recent advances suggest that miniaturised mid-infrared (MIR) devices could replace more time-consuming, laboratory-based techniques for clinical diagnostics. This work uses Fourier transform infrared spectroscopy to show that the MIR complex refractive index of whole blood varies across a range of haematocrit. This indicates that the use of an evanescent measurement is not sufficient to optically exclude the cellular content of blood in the MIR, as previously assumed. Here, spectral refractive index data is presented in two ways. First, it is given as whole blood with varying haematocrit. Second, it is given as the percentage error that haematocrit introduces to plasma. The maximum error in the effective plasma refractive index due to the haematocrit of healthy adults was 0.25% for the real part n and 11% for the imaginary part k. This implies that calibration measurements of haematocrit can be used to account for errors introduced by the cellular content, enabling plasma spectra and analyte concentrations to be indirectly calculated from a whole blood sample. This methodological advance is of clinical importance as plasma concentration of analytes such as drugs can be determined using MIR without the preprocessing of whole blood.

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biosensors-11-00417-v2 - Version of Record
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Accepted/In Press date: 23 October 2021
Published date: 25 October 2021
Keywords: Bioanalytical validation, Biofluid analysis, Mid-infrared spectroscopy, Point-of-care sensing

Identifiers

Local EPrints ID: 452063
URI: http://eprints.soton.ac.uk/id/eprint/452063
ISSN: 2079-6374
PURE UUID: 3e4390ec-7bfc-443c-b498-3b981b79c58e
ORCID for David Rowe: ORCID iD orcid.org/0000-0002-1167-150X
ORCID for Saul Faust: ORCID iD orcid.org/0000-0003-3410-7642
ORCID for James S Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

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Date deposited: 10 Nov 2021 17:33
Last modified: 28 Apr 2022 02:08

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Contributors

Author: David Rowe ORCID iD
Author: Daniel Owens
Author: Suzanne Parker
Author: Saul Faust ORCID iD

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