READ ME File For 'Supporting data_Raw vaules from processed images and biochemical studies'

Dataset DOI: https://doi.org/10.5258/SOTON/D2017

ReadMe Author: Jacqui Taryn Nimmo, University of Southampton

This dataset supports the thesis entitled 'Immunotherapy in Synucleinopathies'
AWARDED BY: Univeristy of Southampton
DATE OF AWARD: 2021

DESCRIPTION OF THE DATA
The dataset contains four types of data:
	1) Raw vaules of percentage area of immunoreactivity from immunohistochemical analysis of images obtained with bright feild or confocal microscopy 
	2) Raw vaules from analysis of western blot obtained from Image Studio.
	3) Raw vaules of blood antibody titres from spectrophotometer readings.
	4) Raw vaules of behaviour tests in mice. 

This dataset contains:

	Image analysis of alpha-synucelin and tau associated with thesis Chapter 3: 
		Bright field_aSyn tau colocalisation analysis.xlsx
		Confocal_aSyn tau colocalisation analysis.xlsx

	Image analysis of drainage of fluorescent tracers (alpha-synucelin and tau) associated with thesis Chapter 4:
		aSyn488_analysis of BV for IPAD_200um from injection site.xlsx
		tau488_analysis of BV for IPAD_200um from injection site.xlsx

	Image analysis of antibodies provided by Vaxxinity associated with thesis Chapter 5:
		Vaxxinity antibody selection IgGs.xlsx
		Vaxxinity antibody selection Lewy bodies.xlsx

	Analysis of images, western blots and antibody titres and behaviour analysis associated with UB312 immunotherapy in thesis Chapter 6:
		UB312_Antibody titre analysis .xlsx
		UB312_Behaviour Analysis.xlsx
		UB312_IHC Analysis.xlsx
		UB312_Native western blot analysis.xlsx
		UB312_Denaturing western blot analysis.xlsx

Date of data collection: [September 2017 - September 2021]

Related projects/Funders:
Funded by MRC and Vaxxinity (formerly named United Neroscience)

Related publication:
Nimmo JT, Johnston DA, Dodart JC, MacGregor-Sharp MT, Weller RO, Nicoll JAR, Verma A, Carare RO (2020) Peri-arterial pathways for clearance of α-Synuclein and tau from the brain: Implications for the pathogenesis of dementias and for immunotherapy. Alzheimer's & dementia (Amsterdam, Netherlands) 12. doi:10.1002/dad2.12070
Nimmo JT, Verma A, Dodart JC, Wang CY, Savistchenko J, Melki R, Carare RO, Nicoll JAR (2020) Novel antibodies detect additional α-synuclein pathology in synucleinopathies: potential development for immunotherapy. Alzheimer's research & therapy 12. doi:10.1186/s13195-020-00727-x
Nimmo JT, Smith H, Wang CY, Teeling J, Nicoll JAR, Verma A, Dodart J-C, Liu Z, Lin F, Carare RO (2021) Immunisation with UB-312 in the Thy1SNCA mouse prevents motor performance deficits and oligomeric α-synuclein accumulation in the brain and gut. Acta Neuropathologica [In Press]



Date that the file was created: October, 2021