Leukotriene B4 limits the effectiveness of fish oil in an animal model of asthma
Leukotriene B4 limits the effectiveness of fish oil in an animal model of asthma
This study aimed to evaluate the levels of eicosanoids derived from arachidonic acid (ARA) in the lungs of asthmatic rats supplemented with fish oil. The present data gives insight into the action of fish oil in asthma, related to its inability to modify the contractile capacity of tracheal smooth muscle reported previously in a model of asthma in rats. Male Wistar rats were supplemented daily with 1 g of fish oil/kg of body weight for 21 days. They were exposed to ovalbumin (OVA) after previous sensitization with OVA to induce asthma. Pulmonary levels of five eicosanoids were measured using immunoassay kits: PGE2, TXB2, LTB4, LXA4, and 8-iso PGF2α. In asthmatic rats, supplementation with fish oil resulted in lower concentrations of lung eicosanoids produced by cyclooxygenase-2 and 15-lipoxygenase: PGE2, TXB2, and LXA4, respectively. Fish oil supplementation also decreased the non-enzymatically produced eicosanoid 8-iso PGF2α. Fish oil supplementation did not affect LTB4, a metabolite of 5-lipoxygenase. The limited efficacy of fish oil supplementation in asthmatic rats is associated with a lack of action in reducing the levels of LTB4 in the lungs. Thus, fish oil differentially modulates the concentrations of eicosanoids derived from ARA via specific pathways in an animal model of asthma.
Asthma, Eicosanoid, Fish oil, Leukotriene
Miranda, D T S Z
0400bc94-1fa3-4531-89a7-2837030a6037
Zanatta, A L
6814af7d-6e67-4554-b761-c5a29d0c8858
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Nishiyama, A
cc446448-832a-48a2-8ef1-a158672f1344
November 2021
Miranda, D T S Z
0400bc94-1fa3-4531-89a7-2837030a6037
Zanatta, A L
6814af7d-6e67-4554-b761-c5a29d0c8858
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Nishiyama, A
cc446448-832a-48a2-8ef1-a158672f1344
Miranda, D T S Z, Zanatta, A L, Miles, Elizabeth, Calder, Philip and Nishiyama, A
(2021)
Leukotriene B4 limits the effectiveness of fish oil in an animal model of asthma.
Heliyon, 7 (11), [e08326].
(doi:10.1016/j.heliyon.2021.e08326).
Abstract
This study aimed to evaluate the levels of eicosanoids derived from arachidonic acid (ARA) in the lungs of asthmatic rats supplemented with fish oil. The present data gives insight into the action of fish oil in asthma, related to its inability to modify the contractile capacity of tracheal smooth muscle reported previously in a model of asthma in rats. Male Wistar rats were supplemented daily with 1 g of fish oil/kg of body weight for 21 days. They were exposed to ovalbumin (OVA) after previous sensitization with OVA to induce asthma. Pulmonary levels of five eicosanoids were measured using immunoassay kits: PGE2, TXB2, LTB4, LXA4, and 8-iso PGF2α. In asthmatic rats, supplementation with fish oil resulted in lower concentrations of lung eicosanoids produced by cyclooxygenase-2 and 15-lipoxygenase: PGE2, TXB2, and LXA4, respectively. Fish oil supplementation also decreased the non-enzymatically produced eicosanoid 8-iso PGF2α. Fish oil supplementation did not affect LTB4, a metabolite of 5-lipoxygenase. The limited efficacy of fish oil supplementation in asthmatic rats is associated with a lack of action in reducing the levels of LTB4 in the lungs. Thus, fish oil differentially modulates the concentrations of eicosanoids derived from ARA via specific pathways in an animal model of asthma.
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Miranda et al. Accepted version
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More information
Accepted/In Press date: 2 November 2021
e-pub ahead of print date: 5 November 2021
Published date: November 2021
Additional Information:
Funding Information:
This research was supported by Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES).
Funding Information:
This research was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) .
Publisher Copyright:
© 2021 The Author(s)
Keywords:
Asthma, Eicosanoid, Fish oil, Leukotriene
Identifiers
Local EPrints ID: 452267
URI: http://eprints.soton.ac.uk/id/eprint/452267
ISSN: 2405-8440
PURE UUID: f6d779d6-8a50-45e9-8457-4e1183e30e07
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Date deposited: 02 Dec 2021 17:34
Last modified: 17 Mar 2024 02:42
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Author:
D T S Z Miranda
Author:
A L Zanatta
Author:
A Nishiyama
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