READ ME File For 'Raw Data for An Investigation Into the Resistance of Spherical Nucleic Acids Against DNA Enzymatic Degradation' 
Dataset DOI: 10.5258/SOTON/D2066 

ReadMe Author: Maria Eleni Kyriazi, University of Southampton

Descritpion:
This dataset supports the publication:

"An Investigation Into the Resistance of Spherical Nucleic Acids Against DNA Enzymatic Degradation"
Maria-Eleni Kyriazi, Afaf H. El-Sagheer, Igor L. Medintz, Tom Brown, Antonios G. Kanaras*
The paper has been accepted for publication in the ACS Journal, Bioconjugate Chemistry.

Contents


All experimental data recorded and used to generate the above title manuscript and submitted to Bioconjugate Chemistry

In particular the dataset contains the data recorded to generate the following figures included in the publication. 

The figures are as follows:


Fig. 1: Quantitative assessment of gold nanoparticle surgace functionalization 

Fig. 2:  Percentage of oligonucleotides remaining on the nanoparticle surface after incubation with DNase I (A) and DNase II (B)

Fig. 3: Percentage of oligonucleotides remaining on the gold nanoparticle surface as a function of oligonucleotide loading per gold nanoparticle after treatment with either DNase I (A) or DNase II (B) for 24 h 

Fig. 4: Quantitative assessment of PS oligonucleotide loading

Fig. 5: Percentage of PS oligonucleotides remaining after a 24 h incubation of PS SNAs with either DNase I (A) or DNase II (B)

Fig. 6: Confocal microscopy images of MRC5 cells treated with DNA coated oligonucleotides

Fig. S1: Representative transmission electron microscopy (TEM) image and UV-Vis spectrum of spherical gold nanoparticles

Fig. S2: UV-Vis spectra of AuNPs functionalised with a varying density of unmodified oligonucleotides in phosphate buffer saline (PBS)

Fig S3: Percentage of oligonucleotides remaining on the gold nanoparticle surface over different time intervals for each oligonucleotide desnity after incubation with DNase I (A) or DNase II (B)

Fig S4:PAGE gel electrophoresis used to determine the efficiency of phosphorothioate modified oligonucleotides towards resisting degradation in the presence of DNase I (A) or DNase II (B)

Fig S5: Fluorescence analysis of PS SNA sense/flare hybridization

Fig S6: SNA response following incubation with a perfect target

Fig S7: . UV-Vis spectra of AuNPs functionalised with a varying density of PS oligonucleotides in PBS



Geographic location of data collection: University of Southampton, U.K.

Related projects:BB/N021150/1

Dataset available under a CC BY 4.0 licence


Publisher: University of Southampton, U.K.


Date: December 2021