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Postnatal prebiotic supplementation in rats affects adult anxious behaviour, hippocampus, electrophysiology, metabolomics, and gut microbiota

Postnatal prebiotic supplementation in rats affects adult anxious behaviour, hippocampus, electrophysiology, metabolomics, and gut microbiota
Postnatal prebiotic supplementation in rats affects adult anxious behaviour, hippocampus, electrophysiology, metabolomics, and gut microbiota

We have shown previously that prebiotic (Bimuno galacto-oligosacharides, B-GOS®) administration to neonatal rats increased hippocampal NMDAR proteins. The present study has investigated the effects of postnatal B-GOS® supplementation on hippocampus-dependent behavior in young, adolescent, and adult rats and applied electrophysiological, metabolomic and metagenomic analyses to explore potential underlying mechanisms. The administration of B-GOS® to suckling, but not post-weaned, rats reduced anxious behavior until adulthood. Neonatal prebiotic intake also reduced the fast decay component of hippocampal NMDAR currents, altered age-specific trajectories of the brain, intestinal, and liver metabolomes, and reduced abundance of fecal Enterococcus and Dorea bacteria. Our data are the first to show that prebiotic administration to rats during a specific postnatal period has long-term effects on behavior and hippocampal physiology. The study also suggests that early-life prebiotic intake may affect host brain function through the reduction of stress-related gut bacteria rather than increasing the proliferation of beneficial microbes.

2589-0042
Spitzer, Sonia O
8e475a55-52d4-413d-986b-a4b0e95661c3
Tkacz, Andrzej
86e913f0-7876-483b-b4ae-9f0d2ce5773c
Savignac, Helene M
fc60a09f-cfbe-43a9-a430-16f34f6c201f
Cooper, Matthew
e8f307fe-15c1-489f-82b4-439a60b4f089
Giallourou, Natasa
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Mann, Edward O
d958c88e-07db-4b14-a61a-bcad01047e7f
Bannerman, David M
c9043df1-8a3c-4784-81a0-09b5ee2975e0
Swann, Jonathan R
7c11a66b-f4b8-4dbf-aa17-ad8b0561b85c
Anthony, Daniel C
928249fa-dcf4-4088-b95b-ce14c719164d
Poole, Philip S
8b49af79-46eb-456a-982e-0944b0d38dbe
Burnet, Philip W J
92663965-a6af-433b-9ea8-a22f1e293275
Spitzer, Sonia O
8e475a55-52d4-413d-986b-a4b0e95661c3
Tkacz, Andrzej
86e913f0-7876-483b-b4ae-9f0d2ce5773c
Savignac, Helene M
fc60a09f-cfbe-43a9-a430-16f34f6c201f
Cooper, Matthew
e8f307fe-15c1-489f-82b4-439a60b4f089
Giallourou, Natasa
b5891ea7-98d4-49d7-b883-2c57ca2d962a
Mann, Edward O
d958c88e-07db-4b14-a61a-bcad01047e7f
Bannerman, David M
c9043df1-8a3c-4784-81a0-09b5ee2975e0
Swann, Jonathan R
7c11a66b-f4b8-4dbf-aa17-ad8b0561b85c
Anthony, Daniel C
928249fa-dcf4-4088-b95b-ce14c719164d
Poole, Philip S
8b49af79-46eb-456a-982e-0944b0d38dbe
Burnet, Philip W J
92663965-a6af-433b-9ea8-a22f1e293275

Spitzer, Sonia O, Tkacz, Andrzej, Savignac, Helene M, Cooper, Matthew, Giallourou, Natasa, Mann, Edward O, Bannerman, David M, Swann, Jonathan R, Anthony, Daniel C, Poole, Philip S and Burnet, Philip W J (2021) Postnatal prebiotic supplementation in rats affects adult anxious behaviour, hippocampus, electrophysiology, metabolomics, and gut microbiota. iScience, 24 (10), [103113]. (doi:10.1016/j.isci.2021.103113).

Record type: Article

Abstract

We have shown previously that prebiotic (Bimuno galacto-oligosacharides, B-GOS®) administration to neonatal rats increased hippocampal NMDAR proteins. The present study has investigated the effects of postnatal B-GOS® supplementation on hippocampus-dependent behavior in young, adolescent, and adult rats and applied electrophysiological, metabolomic and metagenomic analyses to explore potential underlying mechanisms. The administration of B-GOS® to suckling, but not post-weaned, rats reduced anxious behavior until adulthood. Neonatal prebiotic intake also reduced the fast decay component of hippocampal NMDAR currents, altered age-specific trajectories of the brain, intestinal, and liver metabolomes, and reduced abundance of fecal Enterococcus and Dorea bacteria. Our data are the first to show that prebiotic administration to rats during a specific postnatal period has long-term effects on behavior and hippocampal physiology. The study also suggests that early-life prebiotic intake may affect host brain function through the reduction of stress-related gut bacteria rather than increasing the proliferation of beneficial microbes.

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More information

In preparation date: 30 April 2021
Accepted/In Press date: 8 September 2021
e-pub ahead of print date: 10 September 2021
Published date: 22 October 2021
Additional Information: SSRN Electronic Journal (2021) as Pre-Print: DOI: 10.2139/ssrn.3836128 under title Postnatal prebiotic supplementation Has Long-Term Anxiolytic Effects, and Alters Hippocampal Network Activity, Developmental Metabolomics and Gut Microbiota in Male Rats

Identifiers

Local EPrints ID: 453004
URI: http://eprints.soton.ac.uk/id/eprint/453004
ISSN: 2589-0042
PURE UUID: 4ee27c68-0395-4e00-885b-ea19e0dc43ed
ORCID for Jonathan R Swann: ORCID iD orcid.org/0000-0002-6485-4529

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Date deposited: 07 Jan 2022 12:26
Last modified: 17 Mar 2024 04:00

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Contributors

Author: Sonia O Spitzer
Author: Andrzej Tkacz
Author: Helene M Savignac
Author: Matthew Cooper
Author: Natasa Giallourou
Author: Edward O Mann
Author: David M Bannerman
Author: Daniel C Anthony
Author: Philip S Poole
Author: Philip W J Burnet

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