Glycosylation and Serological Reactivity of an Expression-enhanced SARS-CoV-2 Viral Spike Mimetic

Accepted/In Press date: 21 October 2021

e-pub ahead of print date: 27 October 2021

Published date: 30 January 2022

Additional Information: Funding Information: This work was supported by the International AIDS Vaccine Initiative (IAVI) through grant INV-008352/OPP1153692 funded by the Bill and Melinda Gates Foundation (M.C.). We also gratefully acknowledge support from the University of Southampton Coronavirus Response Fund (M.C.), a donation from the Bright Future Trust (M.C.), and the gift of laboratory consumables by Binding Site Ltd. P.J.B. and F.S. gratefully acknowledge support from BII core and grant FY21_CF_HTPO SEED_ID_BII_C211418001 funded by A*STAR. Simulations were performed on petascale computer cluster ASPIRE-1 at the National Supercomputing Centre of Singapore (NSCC), the A*STAR Computational Resource Centre (A*CRC), and the supercomputer Fugaku provided by RIKEN through the HPCI System Research Project (Project ID: hp200303). This work was funded in part by Welch Foundation grant number F-0003-19620604 (J.S.M.). We would like to thank the University of Birmingham Clinical Immunology Service for their invaluable support in sample collection and processing. AFC and AGR are grateful for funding from the Medical Research Council, the Global Challenges Research Fund (GCRF) and the Institute for Global Innovation (IG1 Project, 3107) of the University of Birmingham. We would also like to thank the Wellcome Trust Mechanisms of Inflammatory Disease PhD Programme, 108871/Z/15/Z (R.E.L.). Y.W. has taken up a position at AstraZeneca; all experimental work was performed prior to this development. Funding Information: This work was supported by the International AIDS Vaccine Initiative (IAVI) through grant INV-008352/OPP1153692 funded by the Bill and Melinda Gates Foundation (M.C.). We also gratefully acknowledge support from the University of Southampton Coronavirus Response Fund (M.C.), a donation from the Bright Future Trust (M.C.), and the gift of laboratory consumables by Binding Site Ltd. P.J.B. and F.S. gratefully acknowledge support from BII core and grant FY21_CF_HTPO SEED_ID_BII_C211418001 funded by A*STAR. Simulations were performed on petascale computer cluster ASPIRE-1 at the National Supercomputing Centre of Singapore (NSCC), the A*STAR Computational Resource Centre (A*CRC), and the supercomputer Fugaku provided by RIKEN through the HPCI System Research Project (Project ID: hp200303). This work was funded in part by Welch Foundation grant number F-0003-19620604 (J.S.M.). We would like to thank the University of Birmingham Clinical Immunology Service for their invaluable support in sample collection and processing. AFC and AGR are grateful for funding from the Medical Research Council, the Global Challenges Research Fund (GCRF) and the Institute for Global Innovation (IG1 Project, 3107) of the University of Birmingham. We would also like to thank the Wellcome Trust Mechanisms of Inflammatory Disease PhD Programme, 108871/Z/15/Z (R.E.L.). Y.W. has taken up a position at AstraZeneca; all experimental work was performed prior to this development. Mass spectrometry raw files have been deposited in the MassIVE proteomics databsase, MSV000087945. J.S.M. is an inventor on the following U.S. patent applications: no. 62/412,703 (“Prefusion Coronavirus Spike Proteins and Their Use”); no. 62/972,886 (“2019-nCoV Vaccine”); no. 63/032,502 (“Engineered Coronavirus Spike (S) Protein and Methods of Use Thereof”). Publisher Copyright: © 2021 The Authors

Keywords: SARS-CoV-2, antibody, glycoprotein, glycosylation, serology

Local EPrints ID: 453159

URI: http://eprints.soton.ac.uk/id/eprint/453159

DOI: doi:10.1016/j.jmb.2021.167332

ISSN: 0022-2836

PURE UUID: c2614bb0-b40a-4d41-aa6d-f84ec85c37c2

ORCID for Himanshi Chawla: orcid.org/0000-0001-9828-6593

ORCID for Firdaus Samsudin: orcid.org/0000-0003-2766-4459

ORCID for Joel D Allen: orcid.org/0000-0003-2547-968X

ORCID for Max Crispin: orcid.org/0000-0002-1072-2694

Date deposited: 10 Jan 2022 17:48

Last modified: 17 Mar 2024 04:09