Transcription shifts in gut bacteria shared between mothers and their infants
Transcription shifts in gut bacteria shared between mothers and their infants
The infant gut microbiome contains a portion of bacteria that originate from the maternal gut. In the infant gut these bacteria encounter a new metabolic environment that differs from the adult gut, consequently requiring adjustments in their activities. We used pilot community RNA sequencing data (metatranscriptomes) from ten mother-infant dyads participating in the NiPPeR Study to characterize bacterial gene expression shifts following mother-to-infant transmission. Maternally-derived bacterial strains exhibited large scale gene expression shifts following the transmission to the infant gut, with 12,564 activated and 14,844 deactivated gene families. The implicated genes were most numerous and the magnitude shifts greatest in Bacteroides spp. This pilot study demonstrates environment-dependent, strain-specific shifts in gut bacteria function and underscores the importance of metatranscriptomic analysis in microbiome studies.
Vatanen, T.
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Sakwinska, Olga
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Wilson, B.
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Combremont, S.
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Cutfield, Wayne
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Chan, Shiao-Yng
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Godfrey, Keith
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O'Sullivan, J M
2020b6ff-6dbd-478b-b568-054de94e66ec
24 January 2022
Vatanen, T.
6abdfc22-5720-4ebc-a2e6-11fad91ec67d
Sakwinska, Olga
aa247f71-f435-4a07-81d0-468745037d34
Wilson, B.
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Combremont, S.
e1127b52-846c-4177-a172-27fd201ed085
Cutfield, Wayne
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Chan, Shiao-Yng
3c9d8970-2cc4-430a-86a7-96f6029a5293
Godfrey, Keith
0931701e-fe2c-44b5-8f0d-ec5c7477a6fd
O'Sullivan, J M
2020b6ff-6dbd-478b-b568-054de94e66ec
Vatanen, T., Sakwinska, Olga, Wilson, B., Combremont, S., Cutfield, Wayne, Chan, Shiao-Yng, Godfrey, Keith and O'Sullivan, J M
(2022)
Transcription shifts in gut bacteria shared between mothers and their infants.
Scientific Reports, 12 (1), [1276].
(doi:10.1038/s41598-022-04848-1).
Abstract
The infant gut microbiome contains a portion of bacteria that originate from the maternal gut. In the infant gut these bacteria encounter a new metabolic environment that differs from the adult gut, consequently requiring adjustments in their activities. We used pilot community RNA sequencing data (metatranscriptomes) from ten mother-infant dyads participating in the NiPPeR Study to characterize bacterial gene expression shifts following mother-to-infant transmission. Maternally-derived bacterial strains exhibited large scale gene expression shifts following the transmission to the infant gut, with 12,564 activated and 14,844 deactivated gene families. The implicated genes were most numerous and the magnitude shifts greatest in Bacteroides spp. This pilot study demonstrates environment-dependent, strain-specific shifts in gut bacteria function and underscores the importance of metatranscriptomic analysis in microbiome studies.
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Vatanen SciRep manuscript v2
- Accepted Manuscript
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Accepted/In Press date: 10 December 2021
Published date: 24 January 2022
Additional Information:
Funding Information:
Public good funding for this investigator-led study was through the UK Medical Research Council (as part of an MRC award to the MRC Lifecourse Epidemiology Unit), the Singapore National Research Foundation under its Translational and Clinical Research (TCR) Flagship Programme, the Singapore Government administered through the Agency of Science, Technology and Research (A*STAR; Singapore Institute for Clinical Sciences) and the New Zealand Government (as part of the Gravida, Centre of Research Excellence: Growth and Development). Funding for provision of the intervention and control drinks and to cover aspects of the fieldwork for the NiPPeR study was provided by Société des Produits Nestlé SA under a Research Agreement with the University of Southampton, Auckland UniServices Ltd, Singapore Institute for Clinical Sciences, National University Hospital Singapore PTE Ltd., National University of Singapore. Funding for the microbiome sequencing was provided by Société des Produits Nestlé SA under a Research Agreement with Auckland UniServices Ltd.
Publisher Copyright:
© 2022, The Author(s).
Identifiers
Local EPrints ID: 453178
URI: http://eprints.soton.ac.uk/id/eprint/453178
ISSN: 2045-2322
PURE UUID: 8ceb14ec-3920-4731-9399-cd7e84658967
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Date deposited: 10 Jan 2022 18:00
Last modified: 17 Mar 2024 02:38
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Contributors
Author:
T. Vatanen
Author:
Olga Sakwinska
Author:
B. Wilson
Author:
S. Combremont
Author:
Wayne Cutfield
Author:
Shiao-Yng Chan
Author:
J M O'Sullivan
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