Exposure to low-dose bisphenol A induces spleen damage in a murine model: potentially through oxidative stress?
Exposure to low-dose bisphenol A induces spleen damage in a murine model: potentially through oxidative stress?
Background: During early life, exposure to environmental toxicants, including endocrine disruptor bisphenol A (BPA), can be detrimental to the immune system. To our knowledge, a few researches have looked at the effects of developing BPA exposures on the spleen. Aim: The murine model was developed to investigate the underlying molecular mechanisms and mode of BPA actions on the spleen subsequent to prolonged early-life exposure to BPA. Methods: Immature (3-week-old) male and female Swiss Albino mice were intraperitoneally injected with 50 μg/kg BPA in corn oil or corn oil alone for 6 weeks. Mouse spleens were harvested and examined histologically at 10 weeks old (adulthood). Results: We observed neurobehavioral impairments and a significant increase in peripheral monocyte and lymphocyte counts in mice (males and females). Moreover, several spleen abnormalities in both male and female mice were observed in adulthood. BPA-treated mice’s histopathological results revealed toxicity in the form of significantly active germinal centers of the white pulp and a few apoptotic cells. There was also a notable invasion of the red pulp by eosinophils and lymphocytes that were significantly higher than normal. Agarose gel electrophoresis provided further evidence of internucleosomal DNA fragmentation and apoptosis in the splenic tissues of BPA-treated mice compared to controls. In addition, there were increased levels of the lipid peroxidation malondialdehyde end-product, a marker of oxidative lipid damage, in the spleens of BPA-treated mice compared to controls. Conclusion: Our study provides evidence that oxidative stress injury induced by early-life exposures to BPA could contribute to a range of splenic tissue damages during adulthood.
Apoptosis, Bisphenol A, Murine model, Oxidative stress, Spleen damage
23-32
Shaibi, Taher
73852b14-48d7-4037-86c5-d0806d78ba45
Balug, Hanan N.
b47d7508-4042-4bae-a9fe-c3bd7e843004
Alghazeer, Rabia O.
5acc4581-0ada-46b8-a611-abe68d254b92
Ben-Othman, Mohamed E.
eafb4dde-6e3c-4051-a196-715491b6e50c
Benjama, Ahmeda E.
692a8176-27d7-46dc-9c8b-fdd7b96af48d
Elhensheri, Mohamed
89704237-b1b2-4cbb-9372-6b0a077a7283
Lwaleed, Bashir A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Al-Griw, Mohamed A.
7a5e0326-8d98-4f08-ad27-0f24a0227d41
7 January 2022
Shaibi, Taher
73852b14-48d7-4037-86c5-d0806d78ba45
Balug, Hanan N.
b47d7508-4042-4bae-a9fe-c3bd7e843004
Alghazeer, Rabia O.
5acc4581-0ada-46b8-a611-abe68d254b92
Ben-Othman, Mohamed E.
eafb4dde-6e3c-4051-a196-715491b6e50c
Benjama, Ahmeda E.
692a8176-27d7-46dc-9c8b-fdd7b96af48d
Elhensheri, Mohamed
89704237-b1b2-4cbb-9372-6b0a077a7283
Lwaleed, Bashir A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Al-Griw, Mohamed A.
7a5e0326-8d98-4f08-ad27-0f24a0227d41
Shaibi, Taher, Balug, Hanan N., Alghazeer, Rabia O., Ben-Othman, Mohamed E., Benjama, Ahmeda E., Elhensheri, Mohamed, Lwaleed, Bashir A. and Al-Griw, Mohamed A.
(2022)
Exposure to low-dose bisphenol A induces spleen damage in a murine model: potentially through oxidative stress?
Open Veterinary Journal, 12 (1), .
(doi:10.5455/OVJ.2022.v12.i1.4).
Abstract
Background: During early life, exposure to environmental toxicants, including endocrine disruptor bisphenol A (BPA), can be detrimental to the immune system. To our knowledge, a few researches have looked at the effects of developing BPA exposures on the spleen. Aim: The murine model was developed to investigate the underlying molecular mechanisms and mode of BPA actions on the spleen subsequent to prolonged early-life exposure to BPA. Methods: Immature (3-week-old) male and female Swiss Albino mice were intraperitoneally injected with 50 μg/kg BPA in corn oil or corn oil alone for 6 weeks. Mouse spleens were harvested and examined histologically at 10 weeks old (adulthood). Results: We observed neurobehavioral impairments and a significant increase in peripheral monocyte and lymphocyte counts in mice (males and females). Moreover, several spleen abnormalities in both male and female mice were observed in adulthood. BPA-treated mice’s histopathological results revealed toxicity in the form of significantly active germinal centers of the white pulp and a few apoptotic cells. There was also a notable invasion of the red pulp by eosinophils and lymphocytes that were significantly higher than normal. Agarose gel electrophoresis provided further evidence of internucleosomal DNA fragmentation and apoptosis in the splenic tissues of BPA-treated mice compared to controls. In addition, there were increased levels of the lipid peroxidation malondialdehyde end-product, a marker of oxidative lipid damage, in the spleens of BPA-treated mice compared to controls. Conclusion: Our study provides evidence that oxidative stress injury induced by early-life exposures to BPA could contribute to a range of splenic tissue damages during adulthood.
Text
Exposure to low-dose bisphenol A induces spleen damage in a murine model - potentially through oxidative stress
- Accepted Manuscript
More information
Accepted/In Press date: 29 November 2021
Published date: 7 January 2022
Keywords:
Apoptosis, Bisphenol A, Murine model, Oxidative stress, Spleen damage
Identifiers
Local EPrints ID: 453195
URI: http://eprints.soton.ac.uk/id/eprint/453195
ISSN: 2226-4485
PURE UUID: 1d0f6649-2567-476c-a23a-2b8781713f76
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Date deposited: 10 Jan 2022 18:04
Last modified: 12 Jun 2026 01:39
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Contributors
Author:
Taher Shaibi
Author:
Hanan N. Balug
Author:
Rabia O. Alghazeer
Author:
Mohamed E. Ben-Othman
Author:
Ahmeda E. Benjama
Author:
Mohamed Elhensheri
Author:
Mohamed A. Al-Griw
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