he use of the neutral red retention assay to examine the effects of temperature and salinity on haemocytes of the European flat oyster Ostrea edulis (L)

Hawkins, Lawrence, Hauton, Christopher and Hutchinson, S. (1998) he use of the neutral red retention assay to examine the effects of temperature and salinity on haemocytes of the European flat oyster Ostrea edulis (L). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 119 (4), 619-623. (doi:10.1016/S0305-0491(98)00036-4).

Record type: Article

Abstract

The neutral red retention assay was adapted to assess the effects of a matrix of temperature and salinity combinations on haemocytes of the European flat oyster Ostrea edulis (L.). Oysters were collected from a commercially fished site in the western Solent and were acclimated to different temperature and salinity combinations in the laboratory. The results indicate that the haemocytes are most stable at high salinity and intermediate temperature (28 parts per thousand/15 degrees C), Furthermore it is evident from the data that at low salinities the adaptive capacity of the cellular processes is reduced to such an extent that the haemocytes are unable to respond to favourable temperature regimes. The use of neutral red as a probe to monitor cellular stability is discussed.

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