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Semi-automation of the polymerase chain reaction for laboratory confirmation of meningococcal disease

Semi-automation of the polymerase chain reaction for laboratory confirmation of meningococcal disease
Semi-automation of the polymerase chain reaction for laboratory confirmation of meningococcal disease

Demand for accurate high-throughput detection and characterisation of medically important bacteria has increased dramatically within research and clinical laboratories. Liquid-handling robots have been developed to achieve high levels of accuracy and reproducibility. Assay automation can play a key role in the modern diagnostic laboratory and the data presented here shows that automated PCR is comparable with manual methods. Importantly, automation is preferred when high-quality results cannot be guaranteed using manual methods. This is particularly important when results are required quickly for public health management.

Bacteriological Techniques/instrumentation, DNA, Bacterial/analysis, Humans, Meningitis, Meningococcal/diagnosis, Neisseria meningitidis/isolation & purification, Polymerase Chain Reaction/instrumentation, Robotics/instrumentation
0967-4845
137-40
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17

Diggle, M A and Clarke, S C (2002) Semi-automation of the polymerase chain reaction for laboratory confirmation of meningococcal disease. British Journal of Biomedical Science, 59 (3), 137-40. (doi:10.1080/09674845.2002.11783649).

Record type: Article

Abstract

Demand for accurate high-throughput detection and characterisation of medically important bacteria has increased dramatically within research and clinical laboratories. Liquid-handling robots have been developed to achieve high levels of accuracy and reproducibility. Assay automation can play a key role in the modern diagnostic laboratory and the data presented here shows that automated PCR is comparable with manual methods. Importantly, automation is preferred when high-quality results cannot be guaranteed using manual methods. This is particularly important when results are required quickly for public health management.

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More information

Published date: 2002
Keywords: Bacteriological Techniques/instrumentation, DNA, Bacterial/analysis, Humans, Meningitis, Meningococcal/diagnosis, Neisseria meningitidis/isolation & purification, Polymerase Chain Reaction/instrumentation, Robotics/instrumentation

Identifiers

Local EPrints ID: 453836
URI: http://eprints.soton.ac.uk/id/eprint/453836
ISSN: 0967-4845
PURE UUID: a9a2fe78-bb73-4fe9-a37f-e5ed05d04418
ORCID for S C Clarke: ORCID iD orcid.org/0000-0002-7009-1548

Catalogue record

Date deposited: 25 Jan 2022 17:30
Last modified: 23 Jul 2022 01:56

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Contributors

Author: M A Diggle
Author: S C Clarke ORCID iD

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