Semi-automation of the polymerase chain reaction for laboratory confirmation of meningococcal disease
Semi-automation of the polymerase chain reaction for laboratory confirmation of meningococcal disease
Demand for accurate high-throughput detection and characterisation of medically important bacteria has increased dramatically within research and clinical laboratories. Liquid-handling robots have been developed to achieve high levels of accuracy and reproducibility. Assay automation can play a key role in the modern diagnostic laboratory and the data presented here shows that automated PCR is comparable with manual methods. Importantly, automation is preferred when high-quality results cannot be guaranteed using manual methods. This is particularly important when results are required quickly for public health management.
Bacteriological Techniques/instrumentation, DNA, Bacterial/analysis, Humans, Meningitis, Meningococcal/diagnosis, Neisseria meningitidis/isolation & purification, Polymerase Chain Reaction/instrumentation, Robotics/instrumentation
137-40
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
2002
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A and Clarke, S C
(2002)
Semi-automation of the polymerase chain reaction for laboratory confirmation of meningococcal disease.
British Journal of Biomedical Science, 59 (3), .
(doi:10.1080/09674845.2002.11783649).
Abstract
Demand for accurate high-throughput detection and characterisation of medically important bacteria has increased dramatically within research and clinical laboratories. Liquid-handling robots have been developed to achieve high levels of accuracy and reproducibility. Assay automation can play a key role in the modern diagnostic laboratory and the data presented here shows that automated PCR is comparable with manual methods. Importantly, automation is preferred when high-quality results cannot be guaranteed using manual methods. This is particularly important when results are required quickly for public health management.
This record has no associated files available for download.
More information
Published date: 2002
Keywords:
Bacteriological Techniques/instrumentation, DNA, Bacterial/analysis, Humans, Meningitis, Meningococcal/diagnosis, Neisseria meningitidis/isolation & purification, Polymerase Chain Reaction/instrumentation, Robotics/instrumentation
Identifiers
Local EPrints ID: 453836
URI: http://eprints.soton.ac.uk/id/eprint/453836
ISSN: 0967-4845
PURE UUID: a9a2fe78-bb73-4fe9-a37f-e5ed05d04418
Catalogue record
Date deposited: 25 Jan 2022 17:30
Last modified: 17 Mar 2024 03:07
Export record
Altmetrics
Contributors
Author:
M A Diggle
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
