A novel method for preparing single-stranded DNA for pyrosequencing
A novel method for preparing single-stranded DNA for pyrosequencing
Pyrosequencing technology is a powerful genotyping tool that requires the generation of single stranded DNA. Currently, two simple, solid-phase-based methods are available for this, but they require special equipment, they are not automated, and they are relatively expensive because of the need for biotinylated polymerase chain reaction primers. In this article, an enzymatic liquid-phase method for the generation of high-quality, single-stranded DNA, and its novel use for Pyrosequencing are described. The method has also been fully automated.
Algorithms, DNA, Single-Stranded/genetics, Gene Expression Profiling/methods, Hot Temperature, Neisseria meningitidis/genetics, Polymerase Chain Reaction/methods, Porins/genetics, Robotics/methods, Sequence Analysis, DNA/methods
221-4
Diggle, Matthew A
94088f60-f398-42a0-a221-f71978433987
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
June 2003
Diggle, Matthew A
94088f60-f398-42a0-a221-f71978433987
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, Matthew A and Clarke, Stuart C
(2003)
A novel method for preparing single-stranded DNA for pyrosequencing.
Molecular Biotechnology, 24 (2), .
(doi:10.1385/mb:24:2:221).
Abstract
Pyrosequencing technology is a powerful genotyping tool that requires the generation of single stranded DNA. Currently, two simple, solid-phase-based methods are available for this, but they require special equipment, they are not automated, and they are relatively expensive because of the need for biotinylated polymerase chain reaction primers. In this article, an enzymatic liquid-phase method for the generation of high-quality, single-stranded DNA, and its novel use for Pyrosequencing are described. The method has also been fully automated.
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Published date: June 2003
Keywords:
Algorithms, DNA, Single-Stranded/genetics, Gene Expression Profiling/methods, Hot Temperature, Neisseria meningitidis/genetics, Polymerase Chain Reaction/methods, Porins/genetics, Robotics/methods, Sequence Analysis, DNA/methods
Identifiers
Local EPrints ID: 453847
URI: http://eprints.soton.ac.uk/id/eprint/453847
ISSN: 1073-6085
PURE UUID: 3c5757f1-5176-4283-a62b-a8b97c2f8b53
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Date deposited: 25 Jan 2022 17:38
Last modified: 17 Mar 2024 03:07
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Author:
Matthew A Diggle
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