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A novel method for preparing single-stranded DNA for pyrosequencing

A novel method for preparing single-stranded DNA for pyrosequencing
A novel method for preparing single-stranded DNA for pyrosequencing

Pyrosequencing technology is a powerful genotyping tool that requires the generation of single stranded DNA. Currently, two simple, solid-phase-based methods are available for this, but they require special equipment, they are not automated, and they are relatively expensive because of the need for biotinylated polymerase chain reaction primers. In this article, an enzymatic liquid-phase method for the generation of high-quality, single-stranded DNA, and its novel use for Pyrosequencing are described. The method has also been fully automated.

Algorithms, DNA, Single-Stranded/genetics, Gene Expression Profiling/methods, Hot Temperature, Neisseria meningitidis/genetics, Polymerase Chain Reaction/methods, Porins/genetics, Robotics/methods, Sequence Analysis, DNA/methods
1073-6085
221-4
Diggle, Matthew A
94088f60-f398-42a0-a221-f71978433987
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, Matthew A
94088f60-f398-42a0-a221-f71978433987
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17

Diggle, Matthew A and Clarke, Stuart C (2003) A novel method for preparing single-stranded DNA for pyrosequencing. Molecular Biotechnology, 24 (2), 221-4. (doi:10.1385/mb:24:2:221).

Record type: Article

Abstract

Pyrosequencing technology is a powerful genotyping tool that requires the generation of single stranded DNA. Currently, two simple, solid-phase-based methods are available for this, but they require special equipment, they are not automated, and they are relatively expensive because of the need for biotinylated polymerase chain reaction primers. In this article, an enzymatic liquid-phase method for the generation of high-quality, single-stranded DNA, and its novel use for Pyrosequencing are described. The method has also been fully automated.

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More information

Published date: June 2003
Keywords: Algorithms, DNA, Single-Stranded/genetics, Gene Expression Profiling/methods, Hot Temperature, Neisseria meningitidis/genetics, Polymerase Chain Reaction/methods, Porins/genetics, Robotics/methods, Sequence Analysis, DNA/methods

Identifiers

Local EPrints ID: 453847
URI: http://eprints.soton.ac.uk/id/eprint/453847
ISSN: 1073-6085
PURE UUID: 3c5757f1-5176-4283-a62b-a8b97c2f8b53
ORCID for Stuart C Clarke: ORCID iD orcid.org/0000-0002-7009-1548

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Date deposited: 25 Jan 2022 17:38
Last modified: 26 Jan 2022 02:39

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Contributors

Author: Matthew A Diggle
Author: Stuart C Clarke ORCID iD

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