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Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer

Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer
Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer

Multilocus sequence typing (MLST) is used by the Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) as a routine method for the characterization of certain bacterial pathogens. The SMPRL recently started performing MLST on strains of Streptococcus pneumoniae, and here we describe a fully automated method for MLST using a 96-well-format liquid-handling robot and a 96-capillary automated DNA sequencer.

Automation, Bacterial Typing Techniques/methods, Base Sequence, DNA, Bacterial/chemistry, Phenotype, Polymerase Chain Reaction/methods, Streptococcus pneumoniae/genetics
1073-6085
303-8
Jefferies, Johanna
9468e292-0b41-412d-9470-944e257c7bcf
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, Mathew A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Smith, Andrew
f719dbf6-612c-4ecb-9ec8-ae0ac74928eb
Dowson, Chris
7b061646-256d-4b09-8182-e394a481722a
Mitchell, Tim J.
d11e17e1-428b-4b7c-a0f3-4e954ea0562c
Jefferies, Johanna
9468e292-0b41-412d-9470-944e257c7bcf
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, Mathew A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Smith, Andrew
f719dbf6-612c-4ecb-9ec8-ae0ac74928eb
Dowson, Chris
7b061646-256d-4b09-8182-e394a481722a
Mitchell, Tim J.
d11e17e1-428b-4b7c-a0f3-4e954ea0562c

Jefferies, Johanna, Clarke, Stuart C, Diggle, Mathew A, Smith, Andrew, Dowson, Chris and Mitchell, Tim J. (2003) Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer. Molecular Biotechnology, 24 (3), 303-8. (doi:10.1385/MB:24:3:303).

Record type: Article

Abstract

Multilocus sequence typing (MLST) is used by the Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) as a routine method for the characterization of certain bacterial pathogens. The SMPRL recently started performing MLST on strains of Streptococcus pneumoniae, and here we describe a fully automated method for MLST using a 96-well-format liquid-handling robot and a 96-capillary automated DNA sequencer.

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More information

Published date: July 2003
Keywords: Automation, Bacterial Typing Techniques/methods, Base Sequence, DNA, Bacterial/chemistry, Phenotype, Polymerase Chain Reaction/methods, Streptococcus pneumoniae/genetics

Identifiers

Local EPrints ID: 453849
URI: http://eprints.soton.ac.uk/id/eprint/453849
ISSN: 1073-6085
PURE UUID: 67a3e44a-29a7-4ffe-9a66-8bf2388a35cc
ORCID for Stuart C Clarke: ORCID iD orcid.org/0000-0002-7009-1548
ORCID for Andrew Smith: ORCID iD orcid.org/0000-0001-7321-4331

Catalogue record

Date deposited: 25 Jan 2022 17:39
Last modified: 26 Jan 2022 02:39

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Contributors

Author: Johanna Jefferies
Author: Stuart C Clarke ORCID iD
Author: Mathew A Diggle
Author: Andrew Smith ORCID iD
Author: Chris Dowson
Author: Tim J. Mitchell

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