Association of grandmaternal smoking during pregnancy with DNA methylation of grandchildren: the Isle of Wight study
Association of grandmaternal smoking during pregnancy with DNA methylation of grandchildren: the Isle of Wight study
Background: To investigate the intergenerational effects of grandmaternal smoking during pregnancy (GMSDP) on the DNA methylation of grandchildren. Methods: Data from the Isle of Wight birth cohort with information regarding GMSDP and DNA methylation profiling at the birth of grandchildren (n = 161) were used. Differentially methylated CpG sites related to GMSDP were identified using testing-training screening, analysis of variance and multivariate analysis of covariance. The association between identified CpG sites and expression levels of neighboring genes was tested by linear regression. Results: Twenty-three CpG sites were differentially methylated in grandchildren because of GMSDP, and eight of these were associated with expression levels of 13 neighboring genes. Conclusion: GMSDP has an intergenerational effect on the DNA methylation profile of grandchildren independent of maternal smoking during pregnancy.
DNA methylation, grandchildren, grandmaternal smoking during pregnancy, intergenerational effect, smoking
1473-1483
Luo, Rui
fd323ddc-9115-415e-87d9-594bfde6c90d
Zhang, Hongmei
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Mukherjee, Nandini
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Karmaus, Wilfried
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Patil, Veeresh
25d44602-ebc7-4487-879a-c50500be203a
Arshad, Hasan
917e246d-2e60-472f-8d30-94b01ef28958
Mzayek, Fawaz
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1 October 2021
Luo, Rui
fd323ddc-9115-415e-87d9-594bfde6c90d
Zhang, Hongmei
9f774048-54d6-4321-a252-3887b2c76db0
Mukherjee, Nandini
f64f02d6-2fd0-40db-88ee-5f85b59b8e0b
Karmaus, Wilfried
281d0e53-6b5d-4d38-9732-3981b07cd853
Patil, Veeresh
25d44602-ebc7-4487-879a-c50500be203a
Arshad, Hasan
917e246d-2e60-472f-8d30-94b01ef28958
Mzayek, Fawaz
0e635b70-c9f4-4cb0-a334-c75bb401559c
Luo, Rui, Zhang, Hongmei, Mukherjee, Nandini, Karmaus, Wilfried, Patil, Veeresh, Arshad, Hasan and Mzayek, Fawaz
(2021)
Association of grandmaternal smoking during pregnancy with DNA methylation of grandchildren: the Isle of Wight study.
Epigenomics, 13 (18), .
(doi:10.2217/epi-2020-0433).
Abstract
Background: To investigate the intergenerational effects of grandmaternal smoking during pregnancy (GMSDP) on the DNA methylation of grandchildren. Methods: Data from the Isle of Wight birth cohort with information regarding GMSDP and DNA methylation profiling at the birth of grandchildren (n = 161) were used. Differentially methylated CpG sites related to GMSDP were identified using testing-training screening, analysis of variance and multivariate analysis of covariance. The association between identified CpG sites and expression levels of neighboring genes was tested by linear regression. Results: Twenty-three CpG sites were differentially methylated in grandchildren because of GMSDP, and eight of these were associated with expression levels of 13 neighboring genes. Conclusion: GMSDP has an intergenerational effect on the DNA methylation profile of grandchildren independent of maternal smoking during pregnancy.
Text
Association of GMSDP with grandchildren DNA methylation-preprint version
- Accepted Manuscript
More information
Accepted/In Press date: 10 September 2021
Published date: 1 October 2021
Additional Information:
Funding Information:
This study was funded by the NIH (grant number: R01 AI091905-01). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.
Publisher Copyright:
© 2021
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
Keywords:
DNA methylation, grandchildren, grandmaternal smoking during pregnancy, intergenerational effect, smoking
Identifiers
Local EPrints ID: 453862
URI: http://eprints.soton.ac.uk/id/eprint/453862
ISSN: 1750-1911
PURE UUID: 3c70658a-88e1-48c9-854e-14bf9248ba2c
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Date deposited: 25 Jan 2022 17:41
Last modified: 17 Mar 2024 07:00
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Contributors
Author:
Rui Luo
Author:
Hongmei Zhang
Author:
Nandini Mukherjee
Author:
Wilfried Karmaus
Author:
Veeresh Patil
Author:
Fawaz Mzayek
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