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AXR4 Is Required for Localization of the Auxin Influx Facilitator AUX1

AXR4 Is Required for Localization of the Auxin Influx Facilitator AUX1
AXR4 Is Required for Localization of the Auxin Influx Facilitator AUX1
The AUX1 and PIN auxin influx and efflux facilitators are key regulators of root growth and development. For root gravitropism to occur, AUX1 and PIN2 must transport auxin via the lateral root cap to elongating epidermal cells. Genetic studies suggest that AXR4 functions in the same pathway as AUX1. Here we show that AXR4 is a previously unidentified accessory protein of the endoplasmic reticulum (ER) that regulates localization of AUX1 but not of PIN proteins. Loss of AXR4 resulted in abnormal accumulation of AUX1 in the ER of epidermal cells, indicating that the axr4 agravitropic phenotype is caused by defective AUX1 trafficking in the root epidermis.
0036-8075
1218-1220
Dharmasiri, S.
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Swarup, R.
898e93a2-5602-4589-b9f7-70306e736016
Mockaitis, K.
068c8161-0d88-48ce-bd1f-7842b32f3a43
Dharmasiri, N.
a23fabcc-da9e-4a6f-ba0f-218b3d6cb865
Singh, S.K.
eb887ad5-a055-4929-9690-17ce7c3209e0
Kowalchyk, M.
1bf80d3e-8b97-42cc-925d-4374b32dd526
Marchant, A.
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Mills, S.
e62e70ad-5e1d-4e51-a57e-028a6d412df2
Sandberg, G.
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Benett, M.J.
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Estelle, M.
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Dharmasiri, S.
eb833755-cb1f-4b33-998d-140e6af9eef0
Swarup, R.
898e93a2-5602-4589-b9f7-70306e736016
Mockaitis, K.
068c8161-0d88-48ce-bd1f-7842b32f3a43
Dharmasiri, N.
a23fabcc-da9e-4a6f-ba0f-218b3d6cb865
Singh, S.K.
eb887ad5-a055-4929-9690-17ce7c3209e0
Kowalchyk, M.
1bf80d3e-8b97-42cc-925d-4374b32dd526
Marchant, A.
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Mills, S.
e62e70ad-5e1d-4e51-a57e-028a6d412df2
Sandberg, G.
753924da-94b4-403f-a6f1-b87cb0de3092
Benett, M.J.
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Estelle, M.
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Dharmasiri, S., Swarup, R., Mockaitis, K., Dharmasiri, N., Singh, S.K., Kowalchyk, M., Marchant, A., Mills, S., Sandberg, G., Benett, M.J. and Estelle, M. (2006) AXR4 Is Required for Localization of the Auxin Influx Facilitator AUX1. Science, 312 (5777), 1218-1220. (doi:10.1126/science.1122847).

Record type: Article

Abstract

The AUX1 and PIN auxin influx and efflux facilitators are key regulators of root growth and development. For root gravitropism to occur, AUX1 and PIN2 must transport auxin via the lateral root cap to elongating epidermal cells. Genetic studies suggest that AXR4 functions in the same pathway as AUX1. Here we show that AXR4 is a previously unidentified accessory protein of the endoplasmic reticulum (ER) that regulates localization of AUX1 but not of PIN proteins. Loss of AXR4 resulted in abnormal accumulation of AUX1 in the ER of epidermal cells, indicating that the axr4 agravitropic phenotype is caused by defective AUX1 trafficking in the root epidermis.

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Published date: 26 May 2006

Identifiers

Local EPrints ID: 45415
URI: http://eprints.soton.ac.uk/id/eprint/45415
ISSN: 0036-8075
PURE UUID: 395760a6-77bb-43c7-8728-eb6ba22eab53

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Date deposited: 28 Mar 2007
Last modified: 15 Mar 2024 09:11

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Contributors

Author: S. Dharmasiri
Author: R. Swarup
Author: K. Mockaitis
Author: N. Dharmasiri
Author: S.K. Singh
Author: M. Kowalchyk
Author: A. Marchant
Author: S. Mills
Author: G. Sandberg
Author: M.J. Benett
Author: M. Estelle

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