Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci
Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci
Standard and fluorescence-based PCR assays were developed for the identification of serogroup A meningococci by detection of the mynA gene. This assay was evaluated using bacterial cultures but provides the sensitivity required for the detection of the mynA gene from bodily fluids during meningococcal disease.
Bacterial Proteins/chemistry, Base Sequence, Body Fluids/microbiology, Culture Media, Fluorescent Dyes, Humans, Meningococcal Infections/microbiology, Molecular Sequence Data, Neisseria meningitidis, Serogroup A/classification, Polymerase Chain Reaction/methods, Sensitivity and Specificity, Sequence Analysis, DNA, Species Specificity
1766-1768
Diggle, M A
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Smith, K
a2e75daf-e60c-4548-a4f4-0b2394ffc04a
Girvan, E K
1b33fb56-5546-40e2-8770-2146c91dc300
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
April 2003
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Smith, K
a2e75daf-e60c-4548-a4f4-0b2394ffc04a
Girvan, E K
1b33fb56-5546-40e2-8770-2146c91dc300
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A, Smith, K, Girvan, E K and Clarke, S C
(2003)
Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci.
Journal of Clinical Microbiology, 41 (4), .
(doi:10.1128/JCM.41.4.1766-1768.2003).
Abstract
Standard and fluorescence-based PCR assays were developed for the identification of serogroup A meningococci by detection of the mynA gene. This assay was evaluated using bacterial cultures but provides the sensitivity required for the detection of the mynA gene from bodily fluids during meningococcal disease.
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Published date: April 2003
Keywords:
Bacterial Proteins/chemistry, Base Sequence, Body Fluids/microbiology, Culture Media, Fluorescent Dyes, Humans, Meningococcal Infections/microbiology, Molecular Sequence Data, Neisseria meningitidis, Serogroup A/classification, Polymerase Chain Reaction/methods, Sensitivity and Specificity, Sequence Analysis, DNA, Species Specificity
Identifiers
Local EPrints ID: 454155
URI: http://eprints.soton.ac.uk/id/eprint/454155
ISSN: 0095-1137
PURE UUID: 6ea0ed02-aa6f-45c4-9149-74aca19c849a
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Date deposited: 01 Feb 2022 17:43
Last modified: 17 Mar 2024 03:07
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Author:
M A Diggle
Author:
K Smith
Author:
E K Girvan
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