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Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci

Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci
Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci

Standard and fluorescence-based PCR assays were developed for the identification of serogroup A meningococci by detection of the mynA gene. This assay was evaluated using bacterial cultures but provides the sensitivity required for the detection of the mynA gene from bodily fluids during meningococcal disease.

Bacterial Proteins/chemistry, Base Sequence, Body Fluids/microbiology, Culture Media, Fluorescent Dyes, Humans, Meningococcal Infections/microbiology, Molecular Sequence Data, Neisseria meningitidis, Serogroup A/classification, Polymerase Chain Reaction/methods, Sensitivity and Specificity, Sequence Analysis, DNA, Species Specificity
0095-1137
1766-1768
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Smith, K
a2e75daf-e60c-4548-a4f4-0b2394ffc04a
Girvan, E K
1b33fb56-5546-40e2-8770-2146c91dc300
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Smith, K
a2e75daf-e60c-4548-a4f4-0b2394ffc04a
Girvan, E K
1b33fb56-5546-40e2-8770-2146c91dc300
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17

Diggle, M A, Smith, K, Girvan, E K and Clarke, S C (2003) Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci. Journal of Clinical Microbiology, 41 (4), 1766-1768. (doi:10.1128/JCM.41.4.1766-1768.2003).

Record type: Article

Abstract

Standard and fluorescence-based PCR assays were developed for the identification of serogroup A meningococci by detection of the mynA gene. This assay was evaluated using bacterial cultures but provides the sensitivity required for the detection of the mynA gene from bodily fluids during meningococcal disease.

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More information

Published date: April 2003
Keywords: Bacterial Proteins/chemistry, Base Sequence, Body Fluids/microbiology, Culture Media, Fluorescent Dyes, Humans, Meningococcal Infections/microbiology, Molecular Sequence Data, Neisseria meningitidis, Serogroup A/classification, Polymerase Chain Reaction/methods, Sensitivity and Specificity, Sequence Analysis, DNA, Species Specificity

Identifiers

Local EPrints ID: 454155
URI: http://eprints.soton.ac.uk/id/eprint/454155
ISSN: 0095-1137
PURE UUID: 6ea0ed02-aa6f-45c4-9149-74aca19c849a
ORCID for S C Clarke: ORCID iD orcid.org/0000-0002-7009-1548

Catalogue record

Date deposited: 01 Feb 2022 17:43
Last modified: 02 Feb 2022 02:39

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Contributors

Author: M A Diggle
Author: K Smith
Author: E K Girvan
Author: S C Clarke ORCID iD

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