Comparison of commercial DNA extraction kits for extraction of bacterial genomic DNA from whole-blood samples
Comparison of commercial DNA extraction kits for extraction of bacterial genomic DNA from whole-blood samples
The demand for molecular diagnostic tests in medical microbiology has highlighted the need for efficient methods of DNA extraction. In addition, it is preferable for these methods to be automated. An example of such a requirement is for the confirmation of meningococcal disease where rapid, sensitive, and specific procedures are required for public health management purposes. Previous studies have shown that whole blood is the preferred method for the isolation of bacterial DNA in meningococcal disease, and in this study, we compare five commercially available kits for the extraction of bacterial genomic DNA from whole-blood samples. These include kits in a 96-well binding plate, 96-well filter plate, and metallic bead formats. The method for all five kits is described, and the sensitivity, specificity, ease of automation, and overall efficiency are determined.
Blood/microbiology, DNA, Bacterial/isolation & purification, Genome, Bacterial, Haemophilus influenzae/genetics, Humans, Neisseria meningitidis/genetics, Polymerase Chain Reaction/methods, Reagent Kits, Diagnostic, Sensitivity and Specificity, Streptococcus pneumoniae/genetics
2440-3
Smith, K
a2e75daf-e60c-4548-a4f4-0b2394ffc04a
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
June 2003
Smith, K
a2e75daf-e60c-4548-a4f4-0b2394ffc04a
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Smith, K, Diggle, M A and Clarke, S C
(2003)
Comparison of commercial DNA extraction kits for extraction of bacterial genomic DNA from whole-blood samples.
Journal of Clinical Microbiology, 41 (6), .
(doi:10.1128/JCM.41.6.2440-2443.2003).
Abstract
The demand for molecular diagnostic tests in medical microbiology has highlighted the need for efficient methods of DNA extraction. In addition, it is preferable for these methods to be automated. An example of such a requirement is for the confirmation of meningococcal disease where rapid, sensitive, and specific procedures are required for public health management purposes. Previous studies have shown that whole blood is the preferred method for the isolation of bacterial DNA in meningococcal disease, and in this study, we compare five commercially available kits for the extraction of bacterial genomic DNA from whole-blood samples. These include kits in a 96-well binding plate, 96-well filter plate, and metallic bead formats. The method for all five kits is described, and the sensitivity, specificity, ease of automation, and overall efficiency are determined.
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Published date: June 2003
Keywords:
Blood/microbiology, DNA, Bacterial/isolation & purification, Genome, Bacterial, Haemophilus influenzae/genetics, Humans, Neisseria meningitidis/genetics, Polymerase Chain Reaction/methods, Reagent Kits, Diagnostic, Sensitivity and Specificity, Streptococcus pneumoniae/genetics
Identifiers
Local EPrints ID: 454157
URI: http://eprints.soton.ac.uk/id/eprint/454157
ISSN: 0095-1137
PURE UUID: 5eade7e9-7fcc-4d4d-b2de-5baaf77f2ed3
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Date deposited: 01 Feb 2022 17:43
Last modified: 17 Mar 2024 03:07
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Author:
K Smith
Author:
M A Diggle
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