The use of hydrolysis and hairpin probes in real-time PCR
The use of hydrolysis and hairpin probes in real-time PCR
In this review we discuss the limitations of traditional PCR methods and introduce real-time PCR, a fluorescence-based detection system, as a method that overcomes these limitations. We describe the technologies involved in real-time PCR including the design of hydrolysis and hairpin probes and the practical applications of such systems.
Fluorescence, Fluorescence Resonance Energy Transfer/methods, Fluorescent Dyes/chemistry, Hydrolysis, Oligonucleotide Probes/chemistry, Polymerase Chain Reaction/methods, RNA/analysis, Reproducibility of Results, Sensitivity and Specificity
267-74
McChlery, Susan M
6b06a739-3b31-43f3-ac9d-e08928e9c4cf
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
November 2003
McChlery, Susan M
6b06a739-3b31-43f3-ac9d-e08928e9c4cf
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
McChlery, Susan M and Clarke, Stuart C
(2003)
The use of hydrolysis and hairpin probes in real-time PCR.
Molecular Biotechnology, 25 (3), .
(doi:10.1385/MB:25:3:267).
Abstract
In this review we discuss the limitations of traditional PCR methods and introduce real-time PCR, a fluorescence-based detection system, as a method that overcomes these limitations. We describe the technologies involved in real-time PCR including the design of hydrolysis and hairpin probes and the practical applications of such systems.
This record has no associated files available for download.
More information
Published date: November 2003
Keywords:
Fluorescence, Fluorescence Resonance Energy Transfer/methods, Fluorescent Dyes/chemistry, Hydrolysis, Oligonucleotide Probes/chemistry, Polymerase Chain Reaction/methods, RNA/analysis, Reproducibility of Results, Sensitivity and Specificity
Identifiers
Local EPrints ID: 454172
URI: http://eprints.soton.ac.uk/id/eprint/454172
ISSN: 1073-6085
PURE UUID: e7836644-5791-48ec-af3f-6afed12dcbd0
Catalogue record
Date deposited: 01 Feb 2022 17:57
Last modified: 17 Mar 2024 03:07
Export record
Altmetrics
Contributors
Author:
Susan M McChlery
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
