A single nucleotide polymorphism identification assay for the genotypic characterisation of Neisseria meningitidis using MALDI-TOF mass spectrometry
A single nucleotide polymorphism identification assay for the genotypic characterisation of Neisseria meningitidis using MALDI-TOF mass spectrometry
The ability of matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry (MALDI-TOF) to identify virulent clones of meningococci quickly and accurately is investigated. A single nucleotide polymorphism (SNP) within the fumC gene which differentiates between the hypervirulent ET-15 strain and other ET-37 complex strains is used to determine the usefulness of this method. In this study, MALDI-TOF proved to be a fast, effective alternative to traditional DNA sequencing for the identification of an individual nucleotide.
Bacterial Typing Techniques, Genotype, Humans, Neisseria meningitidis/classification, Polymorphism, Single Nucleotide, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
8-10
Lowe, C A
1e682630-0a78-4a89-9ee7-8a3eb8e4a371
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
2004
Lowe, C A
1e682630-0a78-4a89-9ee7-8a3eb8e4a371
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Lowe, C A, Diggle, M A and Clarke, S C
(2004)
A single nucleotide polymorphism identification assay for the genotypic characterisation of Neisseria meningitidis using MALDI-TOF mass spectrometry.
British Journal of Biomedical Science, 61 (1), .
(doi:10.1080/09674845.2004.11732638).
Abstract
The ability of matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry (MALDI-TOF) to identify virulent clones of meningococci quickly and accurately is investigated. A single nucleotide polymorphism (SNP) within the fumC gene which differentiates between the hypervirulent ET-15 strain and other ET-37 complex strains is used to determine the usefulness of this method. In this study, MALDI-TOF proved to be a fast, effective alternative to traditional DNA sequencing for the identification of an individual nucleotide.
This record has no associated files available for download.
More information
Published date: 2004
Keywords:
Bacterial Typing Techniques, Genotype, Humans, Neisseria meningitidis/classification, Polymorphism, Single Nucleotide, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Identifiers
Local EPrints ID: 454175
URI: http://eprints.soton.ac.uk/id/eprint/454175
ISSN: 0967-4845
PURE UUID: 47b18544-76f7-46cb-abd2-54a64d0910e3
Catalogue record
Date deposited: 01 Feb 2022 17:57
Last modified: 17 Mar 2024 03:07
Export record
Altmetrics
Contributors
Author:
C A Lowe
Author:
M A Diggle
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
