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Pyrosequencing: sequence typing at the speed of light

Pyrosequencing: sequence typing at the speed of light
Pyrosequencing: sequence typing at the speed of light

Nucleotide sequencing is an established method for gaining information relating to partial gene, whole gene, or whole genome sequence. Here we describe some of the background leading to the advent of modern nucleotide sequencing and how it has led to the development of Pyrosequencing, a relatively new method for real-time nucleotide sequencing. In particular, we describe how this method can be used for typing bacterial pathogens.

Bacterial Typing Techniques/methods, Biotechnology/methods, DNA/chemistry, DNA Primers/chemistry, Genes, Bacterial/genetics, Models, Biological, Neisseria meningitidis/genetics, Sequence Analysis, DNA/methods, Software, Time Factors
1073-6085
129-37
Diggle, Matthew A
94088f60-f398-42a0-a221-f71978433987
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, Matthew A
94088f60-f398-42a0-a221-f71978433987
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17

Diggle, Matthew A and Clarke, Stuart C (2004) Pyrosequencing: sequence typing at the speed of light. Molecular Biotechnology, 28 (2), 129-37. (doi:10.1385/mb:28:2:129).

Record type: Review

Abstract

Nucleotide sequencing is an established method for gaining information relating to partial gene, whole gene, or whole genome sequence. Here we describe some of the background leading to the advent of modern nucleotide sequencing and how it has led to the development of Pyrosequencing, a relatively new method for real-time nucleotide sequencing. In particular, we describe how this method can be used for typing bacterial pathogens.

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More information

Published date: October 2004
Keywords: Bacterial Typing Techniques/methods, Biotechnology/methods, DNA/chemistry, DNA Primers/chemistry, Genes, Bacterial/genetics, Models, Biological, Neisseria meningitidis/genetics, Sequence Analysis, DNA/methods, Software, Time Factors

Identifiers

Local EPrints ID: 454178
URI: http://eprints.soton.ac.uk/id/eprint/454178
ISSN: 1073-6085
PURE UUID: 59b32a3c-8986-4aff-b94b-a4adb943f724
ORCID for Stuart C Clarke: ORCID iD orcid.org/0000-0002-7009-1548

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Date deposited: 01 Feb 2022 17:57
Last modified: 17 Mar 2024 03:07

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Contributors

Author: Matthew A Diggle
Author: Stuart C Clarke ORCID iD

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