Genetic analysis of diverse disease-causing pneumococci indicates high levels of diversity within serotypes and capsule switching
Genetic analysis of diverse disease-causing pneumococci indicates high levels of diversity within serotypes and capsule switching
We have used multilocus sequence typing (MLST) and serotyping to build a phylogenetic framework for pneumococcal disease isolates in Scotland that provides a snapshot of the relationships between capsular type and genotype. The results show that while the MLST type correlates with the serotype, isolates within a serotype can belong to a number of individual clonal complexes or sequence types (STs). We also show that isolates of the same ST can express different capsular polysaccharides, i.e., display capsular switching, and that this phenomenon is observed both for capsular types commonly isolated from patients with invasive disease and for serogroups less commonly isolated from patients with invasive disease but which may commonly be carried asymptomatically in the human nasopharynx.
Adolescent, Adult, Aged, Aged, 80 and over, Bacterial Capsules/genetics, Bacterial Typing Techniques, Child, Child, Preschool, Genetic Variation, Humans, Infant, Infant, Newborn, Middle Aged, Pneumococcal Infections/microbiology, Serotyping, Streptococcus pneumoniae/classification
5681-8
Jefferies, Johanna M C
942356b7-2070-49ad-9376-cde03c5fe13d
Smith, Andrew
3e386727-1245-4f1c-9f30-d13bfb63d09d
Clarke, Stuart C
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Dowson, Chris
7b061646-256d-4b09-8182-e394a481722a
Mitchell, Timothy J
56ddfd4d-d6f7-4250-8864-17f156904f95
December 2004
Jefferies, Johanna M C
942356b7-2070-49ad-9376-cde03c5fe13d
Smith, Andrew
3e386727-1245-4f1c-9f30-d13bfb63d09d
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Dowson, Chris
7b061646-256d-4b09-8182-e394a481722a
Mitchell, Timothy J
56ddfd4d-d6f7-4250-8864-17f156904f95
Jefferies, Johanna M C, Smith, Andrew, Clarke, Stuart C, Dowson, Chris and Mitchell, Timothy J
(2004)
Genetic analysis of diverse disease-causing pneumococci indicates high levels of diversity within serotypes and capsule switching.
Journal of Clinical Microbiology, 42 (12), .
(doi:10.1128/JCM.42.12.5681-5688.2004).
Abstract
We have used multilocus sequence typing (MLST) and serotyping to build a phylogenetic framework for pneumococcal disease isolates in Scotland that provides a snapshot of the relationships between capsular type and genotype. The results show that while the MLST type correlates with the serotype, isolates within a serotype can belong to a number of individual clonal complexes or sequence types (STs). We also show that isolates of the same ST can express different capsular polysaccharides, i.e., display capsular switching, and that this phenomenon is observed both for capsular types commonly isolated from patients with invasive disease and for serogroups less commonly isolated from patients with invasive disease but which may commonly be carried asymptomatically in the human nasopharynx.
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Published date: December 2004
Keywords:
Adolescent, Adult, Aged, Aged, 80 and over, Bacterial Capsules/genetics, Bacterial Typing Techniques, Child, Child, Preschool, Genetic Variation, Humans, Infant, Infant, Newborn, Middle Aged, Pneumococcal Infections/microbiology, Serotyping, Streptococcus pneumoniae/classification
Identifiers
Local EPrints ID: 454179
URI: http://eprints.soton.ac.uk/id/eprint/454179
ISSN: 0095-1137
PURE UUID: ed940451-9b12-42d5-923f-7489dd7385fd
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Date deposited: 01 Feb 2022 17:57
Last modified: 17 Mar 2024 03:07
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Author:
Johanna M C Jefferies
Author:
Andrew Smith
Author:
Chris Dowson
Author:
Timothy J Mitchell
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