Transport of Live Cells Under Sterile Conditions Using a Chemotactic Droplet
Transport of Live Cells Under Sterile Conditions Using a Chemotactic Droplet
1-Decanol droplets, formed in an aqueous medium containing decanoate at high pH, become chemotactic when a chemical gradient is placed in the external aqueous environment. We investigated if such droplets can be used as transporters for living cells. We developed a partially hydrophobic alginate capsule as a protective unit that can be precisely placed in a droplet and transported along chemical gradients. Once the droplets with cargo reached a defined final destination, the association of the alginate capsule and decanol droplet was disrupted and cargo deposited. Both Escherichia coli and Bacillus subtilis cells survived and proliferated after transport even though transport occurred under harsh and sterile conditions.
Holler, Silvia
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Porcelli, Carlotta
3187a90c-cace-4659-afeb-e08d289043a9
Ieropoulos, Ioannis A.
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Hanczyc, Martin M.
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30 May 2018
Holler, Silvia
44ff2ecc-8fd8-4b59-9ea5-e23c64a373b8
Porcelli, Carlotta
3187a90c-cace-4659-afeb-e08d289043a9
Ieropoulos, Ioannis A.
6c580270-3e08-430a-9f49-7fbe869daf13
Hanczyc, Martin M.
6ddec5d2-5b32-4f46-abcd-8715adc7ab6f
Holler, Silvia, Porcelli, Carlotta, Ieropoulos, Ioannis A. and Hanczyc, Martin M.
(2018)
Transport of Live Cells Under Sterile Conditions Using a Chemotactic Droplet.
Scientific Reports, 8, [8408].
(doi:10.1038/s41598-018-26703-y).
Abstract
1-Decanol droplets, formed in an aqueous medium containing decanoate at high pH, become chemotactic when a chemical gradient is placed in the external aqueous environment. We investigated if such droplets can be used as transporters for living cells. We developed a partially hydrophobic alginate capsule as a protective unit that can be precisely placed in a droplet and transported along chemical gradients. Once the droplets with cargo reached a defined final destination, the association of the alginate capsule and decanol droplet was disrupted and cargo deposited. Both Escherichia coli and Bacillus subtilis cells survived and proliferated after transport even though transport occurred under harsh and sterile conditions.
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s41598-018-26703-y
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Published date: 30 May 2018
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Local EPrints ID: 454347
URI: http://eprints.soton.ac.uk/id/eprint/454347
ISSN: 2045-2322
PURE UUID: 2162d649-3f30-4a7a-b26b-6d0bbd04c0ab
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Date deposited: 07 Feb 2022 17:53
Last modified: 17 Mar 2024 04:10
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Author:
Silvia Holler
Author:
Carlotta Porcelli
Author:
Martin M. Hanczyc
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