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Genomic-assisted identification of genes involved in secondary growth in Arabidopsis utilising transcript profiling of poplar wood-forming tissues

Record type: Article

Despite the importance of secondary growth in plants, relatively few genes regulating this process have been identified to date. By using data from detailed transcript profiling of the poplar wood-forming tissues, 150 genes that are differentially expressed within the zone of secondary growth were identified. In order to determine the possible function of these poplar genes, potential Arabidopsis thaliana orthologs were identified and gene knockout lines anal- ysed. Three selection filters were used to identify the most likely orthologous genes using poplar and Arabidopsis sequence comparisons, expression pro- filing in secondary thickened Arabidopsis hypocotyls and global expression analysis of Arabidopsis tissues. Three genes encoding AtCSLA2 (At5g22740), the AtGUT1 GT47 glycosyltransferase (At1g27440) and a protein with no proposed function AtUNKA (At4g27435) were selected for further detailed analysis of their role in secondary growth in Arabidopsis. The presented genome-based approach using both poplar and Arabidopsis systems provides powerful means towards assigning biological functions to enzymes with poorly understood biochemical activity, such as AtCSLA2 and AtGUT1, as well as for proteins with no known function.

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Citation

Ubeda-Tomas, Susana, Edvardsson, Ellinor, Eland, Cathlene, Singh, Kumar, Zadik, Daniel, Aspeborg, Henrik, Gorzsas, Andras, Teeri, Tuula T., Sundberg, Bjorn, Persson, Per, Bennett, Malcolm and Marchant, Alan (2007) Genomic-assisted identification of genes involved in secondary growth in Arabidopsis utilising transcript profiling of poplar wood-forming tissues Physiologia Plantarum, 129, (2), pp. 415-428. (doi:10.1111/j.1399-3054.2006.00817.x).

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Published date: 2007

Identifiers

Local EPrints ID: 45439
URI: http://eprints.soton.ac.uk/id/eprint/45439
ISSN: 0031-9317
PURE UUID: 7fbf99b3-f8bd-4741-bcd5-4f00340fec59

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Date deposited: 29 Mar 2007
Last modified: 17 Jul 2017 15:12

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Contributors

Author: Susana Ubeda-Tomas
Author: Ellinor Edvardsson
Author: Cathlene Eland
Author: Kumar Singh
Author: Daniel Zadik
Author: Henrik Aspeborg
Author: Andras Gorzsas
Author: Tuula T. Teeri
Author: Bjorn Sundberg
Author: Per Persson
Author: Malcolm Bennett
Author: Alan Marchant

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