Modified detergent Ziehl-Neelsen technique for the staining of Cyclospora cayetanensis
Modified detergent Ziehl-Neelsen technique for the staining of Cyclospora cayetanensis
Cyclospora cayetanensis is a cause of prolonged diarrhoea, mainly in travellers. Laboratory diagnosis may be achieved by a number of methods such as the staining of faecal smears by the modified Ziehl-Neelsen (ZN) technique. Safer methods using this technique have been described for the staining of acid fast bacilli and cryptosporidia by replacing the phenol content of the carbol fuschin stain with various concentrated detergents. In this report the technique was modified slightly using a non-concentrated detergent and applied to the staining of oocysts of C cayetanensis. It was found that oocysts of C cayetanensis do not stain using the modified detergent ZN method when compared with similar preparations containing oocysts of Cryptosporidium spp.
Animals, Cryptosporidium/isolation & purification, Eucoccidiida/isolation & purification, Staining and Labeling
511-2
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
McIntyre, M
8f8b7e5a-65db-4f8c-ad59-63b524ed59b1
June 1996
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
McIntyre, M
8f8b7e5a-65db-4f8c-ad59-63b524ed59b1
Clarke, S C and McIntyre, M
(1996)
Modified detergent Ziehl-Neelsen technique for the staining of Cyclospora cayetanensis.
Journal of Clinical Pathology, 49 (6), .
(doi:10.1136/jcp.49.6.511).
Abstract
Cyclospora cayetanensis is a cause of prolonged diarrhoea, mainly in travellers. Laboratory diagnosis may be achieved by a number of methods such as the staining of faecal smears by the modified Ziehl-Neelsen (ZN) technique. Safer methods using this technique have been described for the staining of acid fast bacilli and cryptosporidia by replacing the phenol content of the carbol fuschin stain with various concentrated detergents. In this report the technique was modified slightly using a non-concentrated detergent and applied to the staining of oocysts of C cayetanensis. It was found that oocysts of C cayetanensis do not stain using the modified detergent ZN method when compared with similar preparations containing oocysts of Cryptosporidium spp.
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Published date: June 1996
Keywords:
Animals, Cryptosporidium/isolation & purification, Eucoccidiida/isolation & purification, Staining and Labeling
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Local EPrints ID: 454897
URI: http://eprints.soton.ac.uk/id/eprint/454897
ISSN: 0021-9746
PURE UUID: 9788dafe-ca8e-418b-a4f4-a9dd86d1fd29
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Date deposited: 01 Mar 2022 17:40
Last modified: 17 Mar 2024 03:07
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Author:
M McIntyre
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