Automation of a fluorescence-based multiplex PCR for the laboratory confirmation of common bacterial pathogens
Automation of a fluorescence-based multiplex PCR for the laboratory confirmation of common bacterial pathogens
A fluorescence-based multiplex PCR was automated for the simultaneous detection of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae in clinical samples from patients with suspected meningitis. Sensitivity of one to two genome copies per 100 microl sample and specificity of 100% for each organism were shown. Automation of DNA extraction, liquid handling, PCR and analysis are achieved on a single platform, which enables a high throughput and rapid turnaround of clinical samples that, in turn, leads to faster diagnosis. This is ultimately beneficial to the treatment of the patient and for public health management.
Adolescent, Adult, Aged, Aged, 80 and over, Automation, Child, Child, Preschool, DNA, Bacterial/analysis, Fluorescence, Haemophilus influenzae/genetics, Humans, Infant, Infant, Newborn, Meningitis, Bacterial/diagnosis, Middle Aged, Neisseria meningitidis/genetics, Polymerase Chain Reaction/instrumentation, Robotics, Sensitivity and Specificity, Streptococcus pneumoniae/genetics
115-117
Smith, Karen
8ab435e1-c13c-46e5-ab70-a1ca6f543fcc
Diggle, Mathew A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
February 2004
Smith, Karen
8ab435e1-c13c-46e5-ab70-a1ca6f543fcc
Diggle, Mathew A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Clarke, Stuart C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Smith, Karen, Diggle, Mathew A and Clarke, Stuart C
(2004)
Automation of a fluorescence-based multiplex PCR for the laboratory confirmation of common bacterial pathogens.
Journal of Medical Microbiology, 53 (Pt 2), .
(doi:10.1099/jmm.0.05416-0).
Abstract
A fluorescence-based multiplex PCR was automated for the simultaneous detection of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae in clinical samples from patients with suspected meningitis. Sensitivity of one to two genome copies per 100 microl sample and specificity of 100% for each organism were shown. Automation of DNA extraction, liquid handling, PCR and analysis are achieved on a single platform, which enables a high throughput and rapid turnaround of clinical samples that, in turn, leads to faster diagnosis. This is ultimately beneficial to the treatment of the patient and for public health management.
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More information
Published date: February 2004
Keywords:
Adolescent, Adult, Aged, Aged, 80 and over, Automation, Child, Child, Preschool, DNA, Bacterial/analysis, Fluorescence, Haemophilus influenzae/genetics, Humans, Infant, Infant, Newborn, Meningitis, Bacterial/diagnosis, Middle Aged, Neisseria meningitidis/genetics, Polymerase Chain Reaction/instrumentation, Robotics, Sensitivity and Specificity, Streptococcus pneumoniae/genetics
Identifiers
Local EPrints ID: 454971
URI: http://eprints.soton.ac.uk/id/eprint/454971
ISSN: 0022-2615
PURE UUID: 3f5107d6-b929-4f8e-b758-827d08319dfb
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Date deposited: 02 Mar 2022 18:02
Last modified: 17 Mar 2024 03:07
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Contributors
Author:
Karen Smith
Author:
Mathew A Diggle
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