Transdermal delivery of insulin across human skin in vitro with 3D printed hollow microneedles
Transdermal delivery of insulin across human skin in vitro with 3D printed hollow microneedles
In the current study hollow microneedles (HMNs) were fabricated by means of vat polymerization method for the transdermal delivery of insulin. Two geometries of HMNs were designed in a Computer Aided Design (CAD) software namely, curved pyramid and syringe-like and fabricated with Liquid Crystal Display (LCD) method. Dimensions were determined and quality features were imaged with scanning electron microscopy (SEM). Volumetric characterization of HMNs and microchannels was performed by microfocus computed tomography (μCT) whereas mechanical characterization and skin penetration tests of the two geometries were carried out both experimentally and by Finite Element Analysis (FEA) simulation. Diffusion studies of insulin across full thickness human skin were performed in vitro using Franz diffusion cells. Insulin samples were analyzed with liquid chromatography-mass spectrometry (LC-MS). The results show that the transport might be affected by the shape of the microneedles.
3D printed hollow microneedles, Insulin, Transdermal delivery
Xenikakis, Iakovos
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Tsongas, Konstantinos
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Tzimtzimis, Emmanouil K.
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Katsamenis, Orestis
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Demiri, Efterpi
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Zacharis, Constantinos K.
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Georgiou, Despoina
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Kalogianni, Eleni P.
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Tzetzis, Dimitrios
3b55f4b1-0bcc-4237-b1ba-3108c42fc6c9
Fatouros, Dimitrios G.
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January 2022
Xenikakis, Iakovos
3885f841-aa8d-4aca-8cd9-0350941888a1
Tsongas, Konstantinos
fecf477a-84e6-454b-8bd3-9d645d60f2c6
Tzimtzimis, Emmanouil K.
3e043894-d2ad-4069-84f6-3f166af70b44
Katsamenis, Orestis
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Demiri, Efterpi
7ab34de2-2618-4c13-b619-d9410c66c5f8
Zacharis, Constantinos K.
0f09a9fe-0bea-4ac2-a93a-51fc3d6bb1a5
Georgiou, Despoina
9e43ab50-5109-47b7-a14a-70817e5577ae
Kalogianni, Eleni P.
a4497826-af8a-4472-9543-4e8ede8f2436
Tzetzis, Dimitrios
3b55f4b1-0bcc-4237-b1ba-3108c42fc6c9
Fatouros, Dimitrios G.
4a716c96-a8ba-4fbf-b3db-3b2a3a2794c4
Xenikakis, Iakovos, Tsongas, Konstantinos, Tzimtzimis, Emmanouil K., Katsamenis, Orestis, Demiri, Efterpi, Zacharis, Constantinos K., Georgiou, Despoina, Kalogianni, Eleni P., Tzetzis, Dimitrios and Fatouros, Dimitrios G.
(2022)
Transdermal delivery of insulin across human skin in vitro with 3D printed hollow microneedles.
Journal of Drug Delivery Science and Technology, 67, [102891].
(doi:10.1016/j.jddst.2021.102891).
Abstract
In the current study hollow microneedles (HMNs) were fabricated by means of vat polymerization method for the transdermal delivery of insulin. Two geometries of HMNs were designed in a Computer Aided Design (CAD) software namely, curved pyramid and syringe-like and fabricated with Liquid Crystal Display (LCD) method. Dimensions were determined and quality features were imaged with scanning electron microscopy (SEM). Volumetric characterization of HMNs and microchannels was performed by microfocus computed tomography (μCT) whereas mechanical characterization and skin penetration tests of the two geometries were carried out both experimentally and by Finite Element Analysis (FEA) simulation. Diffusion studies of insulin across full thickness human skin were performed in vitro using Franz diffusion cells. Insulin samples were analyzed with liquid chromatography-mass spectrometry (LC-MS). The results show that the transport might be affected by the shape of the microneedles.
Text
1_s2.0_S1773224721005712_main
- Accepted Manuscript
More information
e-pub ahead of print date: 20 October 2021
Published date: January 2022
Additional Information:
Funding Information:
This research is co-financed by Greece and the European Union (European Social Fund - ESF) through the operational program “Human Resources Development, Education and Lifelong Learning 2014–2020” in the context of the project “Fabrication of 3D printed microneedles for transdermal personalized delivery of insulin” ( MIS 5047927 ). We also acknowledge the μ-VIS X-Ray Imaging Centre at the University of Southampton and Wellcome Trust Biomedical Resource and Technology Development Grant 212940/Z/18/Z for supporting the tomographic imaging studies.
Keywords:
3D printed hollow microneedles, Insulin, Transdermal delivery
Identifiers
Local EPrints ID: 455128
URI: http://eprints.soton.ac.uk/id/eprint/455128
ISSN: 1773-2247
PURE UUID: f9416ba1-4b0b-4683-a367-adc356ec5f26
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Date deposited: 10 Mar 2022 17:33
Last modified: 17 Mar 2024 06:56
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Contributors
Author:
Iakovos Xenikakis
Author:
Konstantinos Tsongas
Author:
Emmanouil K. Tzimtzimis
Author:
Efterpi Demiri
Author:
Constantinos K. Zacharis
Author:
Despoina Georgiou
Author:
Eleni P. Kalogianni
Author:
Dimitrios Tzetzis
Author:
Dimitrios G. Fatouros
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