The University of Southampton
University of Southampton Institutional Repository

Leukotrienes in the sputum and urine of cystic fibrosis children.

Leukotrienes in the sputum and urine of cystic fibrosis children.
Leukotrienes in the sputum and urine of cystic fibrosis children.

1. Leukotrienes (LTs) are potent pro‐inflammatory mediators with actions relevant to the pathophysiology of cystic fibrosis (CF), including increased mucus production, bronchoconstriction, leucocyte chemotaxis, and increased vascular permeability. We have therefore investigated the potential role of LTs in children with CF. Leukotriene E4 levels were assessed in the urine of 30 normal (N) children (aged 1.3‐12.7 years) and 30 CF children (1.6‐14.3 years). Sputum from 13 of the CF children was analysed from LTB4, LTC4, LTD4, and LTE4. LTs were separated by reversed‐phase h.p.l.c. and quantitated by radioimmunoassay. 2. Urinary LTE4 levels were log normally distributed, with geometric mean values (95% confidence intervals) of N: 88.4 (71.3‐ 111) pmol mmol‐1 creatinine (n = 30), and CF: 112 (70.6‐177) pmol mmol‐ 1 creatinine (n = 30; P greater than 0.05). Of the CF subjects, 33% had urinary LTE4 levels above 200 pmol mmol‐1 creatinine, compared with 3.3% of the N children. 3. In sputum, mean (+/‐ s.e. mean) LT concentrations were (pmol g‐1), LTB4: 44.3 +/‐ 10.8, LTC4: 4.9 +/‐ 1.3, LTD4: 1.8 +/‐ 0.9, and LTE4: 67.7 +/‐ 18.9 (n = 13). 4. Urinary LTE4 levels correlated significantly with sputum LTE4 levels (r = 0.673, P = 0.012), and with sputum levels of total cysteinyl‐LTs (r = 0.660, P = 0.014). 5. In conclusion, total cysteinyl‐LT content in sputum is 10‐ fold higher than previously reported, consisting primarily (91%) of LTE4. The high levels of LTE4 and LTB4 in sputum suggest involvement of LTs in the pathophysiology of CF. Urinary LTE4 levels may prove useful as a marker for cysteinyl‐LT production in sputum. 1990 The British Pharmacological Society

0306-5251
861-869
Sampson, AP
74082098-0613-46f3-8838-418c8ecd0fe0
Spencer, DA
c84608fb-1c80-427c-aaa2-671bdb416085
Green, CP
1a5e811c-d35e-49e8-aff9-9086f1203e6f
Piper, PJ
7d88e87e-c5a4-4dcb-b561-89340bd49778
Price, JF
cd93112e-8134-4a5e-a20a-f08f5b587d93
Sampson, AP
74082098-0613-46f3-8838-418c8ecd0fe0
Spencer, DA
c84608fb-1c80-427c-aaa2-671bdb416085
Green, CP
1a5e811c-d35e-49e8-aff9-9086f1203e6f
Piper, PJ
7d88e87e-c5a4-4dcb-b561-89340bd49778
Price, JF
cd93112e-8134-4a5e-a20a-f08f5b587d93

Sampson, AP, Spencer, DA, Green, CP, Piper, PJ and Price, JF (1990) Leukotrienes in the sputum and urine of cystic fibrosis children. British Journal of Clinical Pharmacology, 30 (6), 861-869. (doi:10.1111/j.1365-2125.1990.tb05452.x).

Record type: Article

Abstract

1. Leukotrienes (LTs) are potent pro‐inflammatory mediators with actions relevant to the pathophysiology of cystic fibrosis (CF), including increased mucus production, bronchoconstriction, leucocyte chemotaxis, and increased vascular permeability. We have therefore investigated the potential role of LTs in children with CF. Leukotriene E4 levels were assessed in the urine of 30 normal (N) children (aged 1.3‐12.7 years) and 30 CF children (1.6‐14.3 years). Sputum from 13 of the CF children was analysed from LTB4, LTC4, LTD4, and LTE4. LTs were separated by reversed‐phase h.p.l.c. and quantitated by radioimmunoassay. 2. Urinary LTE4 levels were log normally distributed, with geometric mean values (95% confidence intervals) of N: 88.4 (71.3‐ 111) pmol mmol‐1 creatinine (n = 30), and CF: 112 (70.6‐177) pmol mmol‐ 1 creatinine (n = 30; P greater than 0.05). Of the CF subjects, 33% had urinary LTE4 levels above 200 pmol mmol‐1 creatinine, compared with 3.3% of the N children. 3. In sputum, mean (+/‐ s.e. mean) LT concentrations were (pmol g‐1), LTB4: 44.3 +/‐ 10.8, LTC4: 4.9 +/‐ 1.3, LTD4: 1.8 +/‐ 0.9, and LTE4: 67.7 +/‐ 18.9 (n = 13). 4. Urinary LTE4 levels correlated significantly with sputum LTE4 levels (r = 0.673, P = 0.012), and with sputum levels of total cysteinyl‐LTs (r = 0.660, P = 0.014). 5. In conclusion, total cysteinyl‐LT content in sputum is 10‐ fold higher than previously reported, consisting primarily (91%) of LTE4. The high levels of LTE4 and LTB4 in sputum suggest involvement of LTs in the pathophysiology of CF. Urinary LTE4 levels may prove useful as a marker for cysteinyl‐LT production in sputum. 1990 The British Pharmacological Society

This record has no associated files available for download.

More information

Published date: December 1990

Identifiers

Local EPrints ID: 455263
URI: http://eprints.soton.ac.uk/id/eprint/455263
ISSN: 0306-5251
PURE UUID: c87545b5-4858-44fc-999c-d4b07aabdaf1

Catalogue record

Date deposited: 15 Mar 2022 18:09
Last modified: 15 Mar 2022 18:09

Export record

Altmetrics

Contributors

Author: AP Sampson
Author: DA Spencer
Author: CP Green
Author: PJ Piper
Author: JF Price

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×