The University of Southampton
University of Southampton Institutional Repository

The biotransformation in vitro of cysteinyl leukotrienes in blood of normal and asthmatic subjects

The biotransformation in vitro of cysteinyl leukotrienes in blood of normal and asthmatic subjects
The biotransformation in vitro of cysteinyl leukotrienes in blood of normal and asthmatic subjects

The metabolism of exogenous leukotriene C4 (LTC4), LTD4 and LTE4 (10-8M) was studied in vitro in blood of normal and asthmatic subjects for up to 2 hr by reverse-phase high performance liquid chromatography. In whole blood, incubation of LTC4 (T 1 2 = 11.5 min) resulted in the formation of LTD4 and LTE4 whose biosynthesis was inhibited by serine borate (30 mM). Similar experiments performed with LTD4 (T 1 2 = 5 min) produced a single metabolite (LTE4) which was inhibited by L-cysteine (10mM). On the other hand, LTE4 represented a highly stable product in our in vitro system. The bioconversion of LTC4 or LTD4 was slower in plasma but this effect appeared more pronounced for the cysteinylglycinyl derivative. The bioconversion of LTC4 in 4 whole blood or plasma was almost twice as rapid as LTC4. Experiments performed with asthmitic blood showed no significant difference in the survival of LTC4. These results suggest that blood may play a role in regulating the bioavailability of cysteinyl-containing LTs which could be of relevance to their excretion in man.

0090-6980
425-444
Zakrzewski, J. T.
79eaa1d5-041d-4d6c-9c63-c6d49ee07786
Sampson, A. P.
4ca76f6f-ff35-425d-a7e7-c2bd2ea2df60
Evans, J. M.
Barnes, N. C.
Piper, P. J.
7d88e87e-c5a4-4dcb-b561-89340bd49778
Costello, J. F.
53748fbc-3a08-4ebb-b5db-278bac65c9b1
Zakrzewski, J. T.
79eaa1d5-041d-4d6c-9c63-c6d49ee07786
Sampson, A. P.
4ca76f6f-ff35-425d-a7e7-c2bd2ea2df60
Evans, J. M.
Barnes, N. C.
Piper, P. J.
7d88e87e-c5a4-4dcb-b561-89340bd49778
Costello, J. F.
53748fbc-3a08-4ebb-b5db-278bac65c9b1

Zakrzewski, J. T., Sampson, A. P., Evans, J. M., Barnes, N. C., Piper, P. J. and Costello, J. F. (1989) The biotransformation in vitro of cysteinyl leukotrienes in blood of normal and asthmatic subjects. Prostaglandins, 37 (4), 425-444. (doi:10.1016/0090-6980(89)90093-2).

Record type: Article

Abstract

The metabolism of exogenous leukotriene C4 (LTC4), LTD4 and LTE4 (10-8M) was studied in vitro in blood of normal and asthmatic subjects for up to 2 hr by reverse-phase high performance liquid chromatography. In whole blood, incubation of LTC4 (T 1 2 = 11.5 min) resulted in the formation of LTD4 and LTE4 whose biosynthesis was inhibited by serine borate (30 mM). Similar experiments performed with LTD4 (T 1 2 = 5 min) produced a single metabolite (LTE4) which was inhibited by L-cysteine (10mM). On the other hand, LTE4 represented a highly stable product in our in vitro system. The bioconversion of LTC4 or LTD4 was slower in plasma but this effect appeared more pronounced for the cysteinylglycinyl derivative. The bioconversion of LTC4 in 4 whole blood or plasma was almost twice as rapid as LTC4. Experiments performed with asthmitic blood showed no significant difference in the survival of LTC4. These results suggest that blood may play a role in regulating the bioavailability of cysteinyl-containing LTs which could be of relevance to their excretion in man.

This record has no associated files available for download.

More information

Published date: April 1989

Identifiers

Local EPrints ID: 455266
URI: http://eprints.soton.ac.uk/id/eprint/455266
ISSN: 0090-6980
PURE UUID: 45f2818e-08d0-4229-a805-041bd4d85502

Catalogue record

Date deposited: 15 Mar 2022 18:10
Last modified: 15 Mar 2022 18:10

Export record

Altmetrics

Contributors

Author: J. T. Zakrzewski
Author: A. P. Sampson
Author: J. M. Evans
Author: N. C. Barnes
Author: P. J. Piper
Author: J. F. Costello

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×