Acid-fast bodies in faecal smears stained by the modified Ziehl-Neelsen technique
Acid-fast bodies in faecal smears stained by the modified Ziehl-Neelsen technique
The modified Ziehl-Neelsen (ZN) stain has proved useful in the laboratory diagnosis of cryptosporidiosis and, more recently, for the laboratory diagnosis of cyclospora. Apart from cryptosporidia and cyclospora, many other organisms and artefacts are present in faeces, and may be seen in faecal smears stained by the modified ZN method. Described here is the presence of such organisms and artefacts in faecal samples submitted to the routine microbiology laboratory of a district general hospital. Over 6000 faecal smears were examined using the modified ZN method, with an incidence of cyclospora and cryptosporidium of approximately 0.1% and 1%, respectively. Other organisms and artefacts were observed, with an incidence ranging from 0.1% to 1%. It is emphasised that the identification of known gastrointestinal tract pathogens should not rely solely on the results of their staining reactions. It is essential that criteria such as morphology and size be taken into account to differentiate organisms with similar staining reactions.
Animals, Cryptosporidiosis/diagnosis, Cryptosporidium/isolation & purification, Cyclospora/isolation & purification, Cyclosporiasis/diagnosis, Feces/microbiology, Humans, Rhodophyta/isolation & purification, Spores, Staining and Labeling/methods
7-10
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
McIntyre, M
8f8b7e5a-65db-4f8c-ad59-63b524ed59b1
2001
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
McIntyre, M
8f8b7e5a-65db-4f8c-ad59-63b524ed59b1
Clarke, S C and McIntyre, M
(2001)
Acid-fast bodies in faecal smears stained by the modified Ziehl-Neelsen technique.
British Journal of Biomedical Science, 58 (1), .
Abstract
The modified Ziehl-Neelsen (ZN) stain has proved useful in the laboratory diagnosis of cryptosporidiosis and, more recently, for the laboratory diagnosis of cyclospora. Apart from cryptosporidia and cyclospora, many other organisms and artefacts are present in faeces, and may be seen in faecal smears stained by the modified ZN method. Described here is the presence of such organisms and artefacts in faecal samples submitted to the routine microbiology laboratory of a district general hospital. Over 6000 faecal smears were examined using the modified ZN method, with an incidence of cyclospora and cryptosporidium of approximately 0.1% and 1%, respectively. Other organisms and artefacts were observed, with an incidence ranging from 0.1% to 1%. It is emphasised that the identification of known gastrointestinal tract pathogens should not rely solely on the results of their staining reactions. It is essential that criteria such as morphology and size be taken into account to differentiate organisms with similar staining reactions.
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Published date: 2001
Keywords:
Animals, Cryptosporidiosis/diagnosis, Cryptosporidium/isolation & purification, Cyclospora/isolation & purification, Cyclosporiasis/diagnosis, Feces/microbiology, Humans, Rhodophyta/isolation & purification, Spores, Staining and Labeling/methods
Identifiers
Local EPrints ID: 455314
URI: http://eprints.soton.ac.uk/id/eprint/455314
ISSN: 0967-4845
PURE UUID: 5eadec7b-230f-42a3-9a8b-4565958dc396
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Date deposited: 16 Mar 2022 18:07
Last modified: 17 Mar 2022 02:40
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Author:
M McIntyre
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