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Analysis of PorA variable region 3 in meningococci: implications for vaccine policy?

Analysis of PorA variable region 3 in meningococci: implications for vaccine policy?
Analysis of PorA variable region 3 in meningococci: implications for vaccine policy?

Outer membrane protein (OMP) vaccines are being developed against Neisseria meningitidis serogroup B which may provide protection against common circulating serotypes and serosubtypes in some countries. However, limited data is available in Europe from genosubtyping meningococci. We therefore undertook a retrospective analysis of the three main variable regions, VR1, VR2 as well as VR3, of the porA gene from N. meningitidis isolated from different countries, mainly from Scotland and Sweden. Analysis of this gene showed that, amongst 226 strains studied, there were a total of 78 different strains. No new VR1 or VR2 alleles were found but five new VR3 alleles are described. Our data indicates the importance of analysing the VR3 region of PorA in addition to VR1 and VR2 and also highlights, in general terms, the need for genosubtyping meningococci. Such analyses have major implications for the design of new meningococcal vaccines.

Amino Acid Sequence, Bacterial Vaccines/immunology, Base Sequence, DNA Primers, DNA, Bacterial/genetics, Europe, Gene Amplification, Genotype, Neisseria meningitidis/classification, Porins/chemistry
0264-410X
2468-73
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Mölling, P
fee55d25-4c56-4106-a07a-aa299dcc6f60
Unemo, M
e3c042f0-3e00-4561-885f-7c79cd6d912f
Olcén, P
bb9209c5-5bce-4c15-8993-5d2d83c67255
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Mölling, P
fee55d25-4c56-4106-a07a-aa299dcc6f60
Unemo, M
e3c042f0-3e00-4561-885f-7c79cd6d912f
Olcén, P
bb9209c5-5bce-4c15-8993-5d2d83c67255

Clarke, S C, Diggle, M A, Mölling, P, Unemo, M and Olcén, P (2003) Analysis of PorA variable region 3 in meningococci: implications for vaccine policy? Vaccine, 21 (19-20), 2468-73. (doi:10.1016/S0264-410X(03)00033-1).

Record type: Article

Abstract

Outer membrane protein (OMP) vaccines are being developed against Neisseria meningitidis serogroup B which may provide protection against common circulating serotypes and serosubtypes in some countries. However, limited data is available in Europe from genosubtyping meningococci. We therefore undertook a retrospective analysis of the three main variable regions, VR1, VR2 as well as VR3, of the porA gene from N. meningitidis isolated from different countries, mainly from Scotland and Sweden. Analysis of this gene showed that, amongst 226 strains studied, there were a total of 78 different strains. No new VR1 or VR2 alleles were found but five new VR3 alleles are described. Our data indicates the importance of analysing the VR3 region of PorA in addition to VR1 and VR2 and also highlights, in general terms, the need for genosubtyping meningococci. Such analyses have major implications for the design of new meningococcal vaccines.

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More information

Published date: 2 June 2003
Keywords: Amino Acid Sequence, Bacterial Vaccines/immunology, Base Sequence, DNA Primers, DNA, Bacterial/genetics, Europe, Gene Amplification, Genotype, Neisseria meningitidis/classification, Porins/chemistry

Identifiers

Local EPrints ID: 455315
URI: http://eprints.soton.ac.uk/id/eprint/455315
ISSN: 0264-410X
PURE UUID: ea336ddf-79b7-4925-a737-a3dbc5646872
ORCID for S C Clarke: ORCID iD orcid.org/0000-0002-7009-1548

Catalogue record

Date deposited: 16 Mar 2022 18:07
Last modified: 23 Jul 2022 01:56

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Contributors

Author: S C Clarke ORCID iD
Author: M A Diggle
Author: P Mölling
Author: M Unemo
Author: P Olcén

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