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Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis

Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis
Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis

The Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. Part of this service includes the serogrouping of meningococcal isolates followed by typing and subtyping. The procedures for this are labor-intensive but important for the identification of linked cases and the surveillance of disease so that effective public health measures can be taken. However, different strains of meningococci, such as those within the electrophoretic type 37 complex, occurring during case clusters of disease are now indistinguishable by current methods. The SMPRL has started using multilocus sequence typing (MLST) as a routine method for the characterization of isolates of Neisseria meningitidis. MLST produces nucleotide sequence data of seven housekeeping genes providing results that are useful for public health management. However, the method is laborious and time-consuming and therefore lends itself towards automation. The SMPRL therefore developed a semiautomated method for MLST using a 96-well format liquid handler and an automated DNA sequencer. Semiautomated MLST is now provided as a reference service for Scotland. This work describes the methodology required for the characterization of N. meningitidis and highlights its usefulness for public health intervention.

Automation, Bacterial Typing Techniques/instrumentation, DNA, Bacterial/genetics, Gene Frequency, Genes, Bacterial, Genotype, Humans, Meningococcal Infections/microbiology, Neisseria meningitidis/classification, Phenotype, Porins/genetics, Sequence Analysis, DNA
0095-1137
3066-3071
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Edwards, G F
f98fa562-77bd-45a3-827b-7a3f8dc76cdd
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Edwards, G F
f98fa562-77bd-45a3-827b-7a3f8dc76cdd

Clarke, S C, Diggle, M A and Edwards, G F (2001) Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis. Journal of Clinical Microbiology, 39 (9), 3066-3071. (doi:10.1128/JCM.39.9.3066-3071.2001).

Record type: Article

Abstract

The Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. Part of this service includes the serogrouping of meningococcal isolates followed by typing and subtyping. The procedures for this are labor-intensive but important for the identification of linked cases and the surveillance of disease so that effective public health measures can be taken. However, different strains of meningococci, such as those within the electrophoretic type 37 complex, occurring during case clusters of disease are now indistinguishable by current methods. The SMPRL has started using multilocus sequence typing (MLST) as a routine method for the characterization of isolates of Neisseria meningitidis. MLST produces nucleotide sequence data of seven housekeeping genes providing results that are useful for public health management. However, the method is laborious and time-consuming and therefore lends itself towards automation. The SMPRL therefore developed a semiautomated method for MLST using a 96-well format liquid handler and an automated DNA sequencer. Semiautomated MLST is now provided as a reference service for Scotland. This work describes the methodology required for the characterization of N. meningitidis and highlights its usefulness for public health intervention.

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More information

Published date: September 2001
Keywords: Automation, Bacterial Typing Techniques/instrumentation, DNA, Bacterial/genetics, Gene Frequency, Genes, Bacterial, Genotype, Humans, Meningococcal Infections/microbiology, Neisseria meningitidis/classification, Phenotype, Porins/genetics, Sequence Analysis, DNA

Identifiers

Local EPrints ID: 455383
URI: http://eprints.soton.ac.uk/id/eprint/455383
ISSN: 0095-1137
PURE UUID: ba401ad7-48e5-4b62-8c84-2a050b8a95ba
ORCID for S C Clarke: ORCID iD orcid.org/0000-0002-7009-1548

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Date deposited: 21 Mar 2022 17:31
Last modified: 23 Jul 2022 01:56

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Contributors

Author: S C Clarke ORCID iD
Author: M A Diggle
Author: G F Edwards

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