Introduction of an automated service for the laboratory confirmation of meningococcal disease in Scotland
Introduction of an automated service for the laboratory confirmation of meningococcal disease in Scotland
The Scottish Meningococcus and Pneumococcus Reference Laboratory provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. The main tests used for the laboratory confirmation of meningococcal disease are culture, the polymerase chain reaction (PCR), antibody testing, and more recently DNA sequencing. This paper describes the automation of PCR for the laboratory confirmation of meningococcal disease and the typing of meningococcal isolates using DNA sequencing. Both methods have been automated using a robotic liquid handler and automated DNA sequencer. These methods, along with standard culture phenotyping and antibody testing, provide Scotland with an excellent service for the confirmation of meningococcal disease.
Automation, Bacterial Typing Techniques/methods, Clinical Laboratory Techniques, DNA, Bacterial/genetics, Humans, Meningococcal Infections/diagnosis, Neisseria meningitidis/classification, Polymerase Chain Reaction/methods, Scotland, Sequence Analysis, DNA/methods
556-557
Clarke, S.C.
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Diggle, M.A.
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Reid, J.A.
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Thom, L.
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Edwards, G.F.S.
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Clarke, S.C.
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M.A.
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Reid, J.A.
5c069c99-88a4-4602-ab42-b23c902a52c8
Thom, L.
9ea500f0-d6bb-404b-b6eb-0c55158b968a
Edwards, G.F.S.
f98fa562-77bd-45a3-827b-7a3f8dc76cdd
Clarke, S.C., Diggle, M.A., Reid, J.A., Thom, L. and Edwards, G.F.S.
(2001)
Introduction of an automated service for the laboratory confirmation of meningococcal disease in Scotland.
Journal of Clinical Pathology, 54 (7), .
(doi:10.1136/jcp.54.7.556).
Abstract
The Scottish Meningococcus and Pneumococcus Reference Laboratory provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. The main tests used for the laboratory confirmation of meningococcal disease are culture, the polymerase chain reaction (PCR), antibody testing, and more recently DNA sequencing. This paper describes the automation of PCR for the laboratory confirmation of meningococcal disease and the typing of meningococcal isolates using DNA sequencing. Both methods have been automated using a robotic liquid handler and automated DNA sequencer. These methods, along with standard culture phenotyping and antibody testing, provide Scotland with an excellent service for the confirmation of meningococcal disease.
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More information
Accepted/In Press date: 27 October 2000
e-pub ahead of print date: 1 July 2001
Keywords:
Automation, Bacterial Typing Techniques/methods, Clinical Laboratory Techniques, DNA, Bacterial/genetics, Humans, Meningococcal Infections/diagnosis, Neisseria meningitidis/classification, Polymerase Chain Reaction/methods, Scotland, Sequence Analysis, DNA/methods
Identifiers
Local EPrints ID: 455384
URI: http://eprints.soton.ac.uk/id/eprint/455384
ISSN: 0021-9746
PURE UUID: c3d83b7f-af05-4de6-8f97-377b89e561fa
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Date deposited: 21 Mar 2022 17:31
Last modified: 17 Mar 2024 03:07
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Contributors
Author:
M.A. Diggle
Author:
J.A. Reid
Author:
L. Thom
Author:
G.F.S. Edwards
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