Automated PCR/sequence template purification
Automated PCR/sequence template purification
A commercially available filtration method is described for the purification of polymerase chain reaction (PCR) templates and sequence-labeled products. The methodology is described for the automation of this application and its use on a high-throughput liquid handling robot and capillary-based automated DNA sequencer. The application provides good-quality DNA, is relatively cheap, and can be used in either 96- or 384-well format.
Bacterial Typing Techniques, Electrophoresis/methods, Humans, Micropore Filters, Neisseria meningitidis/classification, Polymerase Chain Reaction/instrumentation, Robotics, Sequence Analysis, DNA, Streptococcus pneumoniae/classification, Templates, Genetic
221-224
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
1 July 2002
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Clarke, S C and Diggle, M A
(2002)
Automated PCR/sequence template purification.
Molecular Biotechnology, 21 (3), .
(doi:10.1385/MB:21:3:221).
Abstract
A commercially available filtration method is described for the purification of polymerase chain reaction (PCR) templates and sequence-labeled products. The methodology is described for the automation of this application and its use on a high-throughput liquid handling robot and capillary-based automated DNA sequencer. The application provides good-quality DNA, is relatively cheap, and can be used in either 96- or 384-well format.
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Published date: 1 July 2002
Keywords:
Bacterial Typing Techniques, Electrophoresis/methods, Humans, Micropore Filters, Neisseria meningitidis/classification, Polymerase Chain Reaction/instrumentation, Robotics, Sequence Analysis, DNA, Streptococcus pneumoniae/classification, Templates, Genetic
Identifiers
Local EPrints ID: 455547
URI: http://eprints.soton.ac.uk/id/eprint/455547
ISSN: 1073-6085
PURE UUID: cc4bc40f-244d-4d3e-8017-bc0d9759d972
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Date deposited: 25 Mar 2022 17:35
Last modified: 17 Mar 2024 03:07
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Author:
M A Diggle
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