Automated non-culture-based sequence typing of meningococci from body fluids
Automated non-culture-based sequence typing of meningococci from body fluids
In recent years, the polymerase chain reaction has been used for the non-culture diagnosis of meningococcal disease, and sequence-based typing takes this further by providing the full characterisation normally only available by culture. In this study, porA gene sequencing was used to perform non-culture-based sequence typing of Neisseria meningitidis strains direct from body fluids. Non-culture porA gene sequencing provided the serosubtype of the infecting organism, and proved to be a useful method as N. meningitidis was not isolated from any of the patients in this study. In conclusion, porA gene sequencing is a very useful tool for the non-culture characterisation of meningococci and provides important information for public health management of cases and contacts.
Bacterial Typing Techniques/methods, Genes, Bacterial, Humans, Neisseria meningitidis/classification, Polymerase Chain Reaction/methods, Porins/genetics, Retrospective Studies
230-4
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Edwards, G F
f98fa562-77bd-45a3-827b-7a3f8dc76cdd
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Edwards, G F
f98fa562-77bd-45a3-827b-7a3f8dc76cdd
Clarke, S C, Diggle, M A and Edwards, G F
(2001)
Automated non-culture-based sequence typing of meningococci from body fluids.
British Journal of Biomedical Science, 58 (4), .
Abstract
In recent years, the polymerase chain reaction has been used for the non-culture diagnosis of meningococcal disease, and sequence-based typing takes this further by providing the full characterisation normally only available by culture. In this study, porA gene sequencing was used to perform non-culture-based sequence typing of Neisseria meningitidis strains direct from body fluids. Non-culture porA gene sequencing provided the serosubtype of the infecting organism, and proved to be a useful method as N. meningitidis was not isolated from any of the patients in this study. In conclusion, porA gene sequencing is a very useful tool for the non-culture characterisation of meningococci and provides important information for public health management of cases and contacts.
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e-pub ahead of print date: 7 September 2001
Keywords:
Bacterial Typing Techniques/methods, Genes, Bacterial, Humans, Neisseria meningitidis/classification, Polymerase Chain Reaction/methods, Porins/genetics, Retrospective Studies
Identifiers
Local EPrints ID: 455549
URI: http://eprints.soton.ac.uk/id/eprint/455549
ISSN: 0967-4845
PURE UUID: c309d3de-6023-4203-b574-07bfd41979e4
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Date deposited: 25 Mar 2022 17:35
Last modified: 17 Mar 2024 03:07
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Author:
M A Diggle
Author:
G F Edwards
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